Медицинская библиотека · Science (New York, N.Y.) · 2020

A cell atlas of human thymic development defines T cell repertoire formation.

Park Jong-Eun, Botting Rachel A, Domínguez Conde Cecilia, Popescu Dorin-Mirel, Lavaert Marieke, Kunz Daniel J, Goh Issac, Stephenson Emily, Ragazzini Roberta, Tuck Elizabeth, Wilbrey-Clark Anna, Roberts Kenny, Kedlian Veronika R, Ferdinand John R, He Xiaoling, Webb Simone, Maunder Daniel, Vandamme Niels, Mahbubani Krishnaa T, Polanski Krzysztof, Mamanova Lira, Bolt Liam, Crossland David, de Rita Fabrizio, Fuller Andrew, Filby Andrew, Reynolds Gary, Dixon David, Saeb-Parsy Kourosh, Lisgo Steven, Henderson Deborah, Vento-Tormo Roser, Bayraktar Omer A, Barker Roger A, Meyer Kerstin B, Saeys Yvan, Bonfanti Paola, Behjati Sam, Clatworthy Menna R, Taghon Tom, Haniffa Muzlifah, Teichmann Sarah A

СтатьяПоддержка исследования: вне правительства СШАПеревод пока не подготовлен

Аннотация

The thymus provides a nurturing environment for the differentiation and selection of T cells, a process orchestrated by their interaction with multiple thymic cell types. We used single-cell RNA sequencing to create a cell census of the human thymus across the life span and to reconstruct T cell differentiation trajectories and T cell receptor (TCR) recombination kinetics. Using this approach, we identified and located in situ CD8αα+ T cell populations, thymic fibroblast subtypes, and activated dendritic cell states. In addition, we reveal a bias in TCR recombination and selection, which is attributed to genomic position and the kinetics of lineage commitment. Taken together, our data provide a comprehensive atlas of the human thymus across the life span with new insights into human T cell development.

Для цитирования: Park Jong-Eun, Botting Rachel A, Domínguez Conde Cecilia, Popescu Dorin-Mirel, Lavaert Marieke, Kunz Daniel J, Goh Issac, Stephenson Emily, Ragazzini Roberta, Tuck Elizabeth, Wilbrey-Clark Anna, Roberts Kenny, Kedlian Veronika R, Ferdinand John R, He Xiaoling, Webb Simone, Maunder Daniel, Vandamme Niels, Mahbubani Krishnaa T, Polanski Krzysztof, Mamanova Lira, Bolt Liam, Crossland David, de Rita Fabrizio, Fuller Andrew, Filby Andrew, Reynolds Gary, Dixon David, Saeb-Parsy Kourosh, Lisgo Steven, Henderson Deborah, Vento-Tormo Roser, Bayraktar Omer A, Barker Roger A, Meyer Kerstin B, Saeys Yvan, Bonfanti Paola, Behjati Sam, Clatworthy Menna R, Taghon Tom, Haniffa Muzlifah, Teichmann Sarah A. A cell atlas of human thymic development defines T cell repertoire formation.. Science (New York, N.Y.). 2020. DOI: 10.1126/science.aay3224.

Предметные рубрики MeSH

Atlases as Topic CD8-Positive T-Lymphocytes Cell Differentiation Dendritic Cells Fibroblasts Humans RNA-Seq Receptors, Antigen, T-Cell Single-Cell Analysis Thymus Gland

Подготовка и перевод публикации

Обработка статьи · этапы, ошибки и управление

Загрузка состояния…

История · было → стало

Состояние обновляется каждые 5 секунд, пока блок открыт. Процент показывается только там, где известен объём работы. Пауза останавливает продолжение этапа; текущий запрос к источнику может завершиться.

Тип публикации указан по данным источника. Уровень доказательности автоматически не присваивается.

Аннотация

Предметные рубрики MeSH

Ошибки и ограничения