Discover oncology · 2026
lncRNA-EBLN3P promotes triple-negative breast cancer metastasis via activating Wnt/β-catenin signaling pathway.
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Cai Ya-Hong, Li Jing-Jing, Bai Guang-Zhen, Fang Can-Ming, Zhao Ye, Liu Jia-Rui, Mei Yi-Jie, Chen Hui-Hui, Xue Rui
Аннотация
INTRODUCTION: To detect the expression of lncRNA endogenous bornavirus-like nucleoprotein (EBLN3P) in triple-negative breast cancer (TNBC) primary cells and tumor tissues. To explore the functional role of EBLN3P in the malignant progression of TNBC.
METHODS: EBLN3P expression was detected by using quantitative reverse transcription polymerase chain reaction (qRT-PCR) and Northern-blot (NB), the influence of EBLN3P to patients´ prognosis was explored by using Kaplan-Meier (K-M) survival analysis. siRNA was used to knockout EBLN3P in TNBC primary cells, 5-ethynyl-2´-deoxyuridine (EdU) and Transwells were used to evaluate changes in proliferation, migration and invasion abilities. RNA-sequence was used to explore changes of signaling pathway.
RESULTS: EBLN3P was aberrantly upregulated in TNBC, especially those patients with distant metastasis. EBLN3P showed a tumor-promoting role, promoted the proliferation, migration and invasion abilities of TNBC by activating Wnt/β-catenin pathway.
CONCLUSION: EBLN3P might be a potential biomarker TNBC, especially for TNBC with distant metastasis.
METHODS: EBLN3P expression was detected by using quantitative reverse transcription polymerase chain reaction (qRT-PCR) and Northern-blot (NB), the influence of EBLN3P to patients´ prognosis was explored by using Kaplan-Meier (K-M) survival analysis. siRNA was used to knockout EBLN3P in TNBC primary cells, 5-ethynyl-2´-deoxyuridine (EdU) and Transwells were used to evaluate changes in proliferation, migration and invasion abilities. RNA-sequence was used to explore changes of signaling pathway.
RESULTS: EBLN3P was aberrantly upregulated in TNBC, especially those patients with distant metastasis. EBLN3P showed a tumor-promoting role, promoted the proliferation, migration and invasion abilities of TNBC by activating Wnt/β-catenin pathway.
CONCLUSION: EBLN3P might be a potential biomarker TNBC, especially for TNBC with distant metastasis.