Tropical animal health and production · 2020
Effect of diluent sugars on capacitation status and acrosome reaction of spermatozoa in buck semen at refrigerated temperature.
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Gangwar Chetna, Kharche Suresh Dinkar, Mishra Anil Kumar, Saraswat Sonia, Kumar Narendra, Sikarwar Anuj Kumar
Аннотация
OBJECTIVE: The aim of the study was to explore the possibility of a better sugar suitable for storage of goat semen at refrigerated temperature.
MATERIALS AND METHOD: For this experiment, semen was collected from eight Jakhrana bucks maintained at Jakhrana unit, ICAR-CIRG, at twice a week interval using artificial vagina. Collected semen was preliminary evaluated, and better semen samples were pooled and divided into two parts. One part of the pooled semen was diluted in egg yolk, Tris, citric acid, and fructose diluter, whereas second part was diluted in egg yolk, Tris, citric acid, and glucose diluter. Then semen samples were kept in equilibration chamber for 4 h at 5 °C after proper dilution. Both the semen samples were evaluated for viability, motility, plasma membrane integrity, sperm abnormality, lipid peroxidation, acrosomal integrity, and capacitation status at 0 h, 24 h, 48 h, and 72 h after dilution.
RESULTS: Significantly (P < 0.05) higher motility was observed at 24 h in extender containing glucose as compared with extender containing fructose but motility was decreased at 48 h and 72 h. Number of capacitated sperm increased significantly (P < 0.05) and acrosomal integrity was decreased significantly (P < 0.05) at 72 h in extender containing glucose. The other parameters like viability and plasma membrane integrity were decreased significantly (P < 0.05) at 72 h and lipid peroxidation as well as sperm abnormality increased significantly (P < 0.05) in extender containing glucose.
CONCLUSION: From this study, it can be concluded that fructose is better diluent sugar for refrigerated storage of buck semen.
MATERIALS AND METHOD: For this experiment, semen was collected from eight Jakhrana bucks maintained at Jakhrana unit, ICAR-CIRG, at twice a week interval using artificial vagina. Collected semen was preliminary evaluated, and better semen samples were pooled and divided into two parts. One part of the pooled semen was diluted in egg yolk, Tris, citric acid, and fructose diluter, whereas second part was diluted in egg yolk, Tris, citric acid, and glucose diluter. Then semen samples were kept in equilibration chamber for 4 h at 5 °C after proper dilution. Both the semen samples were evaluated for viability, motility, plasma membrane integrity, sperm abnormality, lipid peroxidation, acrosomal integrity, and capacitation status at 0 h, 24 h, 48 h, and 72 h after dilution.
RESULTS: Significantly (P < 0.05) higher motility was observed at 24 h in extender containing glucose as compared with extender containing fructose but motility was decreased at 48 h and 72 h. Number of capacitated sperm increased significantly (P < 0.05) and acrosomal integrity was decreased significantly (P < 0.05) at 72 h in extender containing glucose. The other parameters like viability and plasma membrane integrity were decreased significantly (P < 0.05) at 72 h and lipid peroxidation as well as sperm abnormality increased significantly (P < 0.05) in extender containing glucose.
CONCLUSION: From this study, it can be concluded that fructose is better diluent sugar for refrigerated storage of buck semen.