THE INFLUENCE OF CONTRACEPTION ON VAGINAL MICROBIOCENOSIS CONDITION.

Aim - in complex to evaluate different types of female vaginal biocenosis on the background of contraceptives usage in order to detect implant-associated infection. Clinical status, features of vaginal microflora and formation of biofilms were assessed in 64 women of reproductive age, who had inert intrauterine device (IUD) of the second generation for a period of from 5 months to 8 years. pH of vaginal secretion was measured by the test strips CITOLAB (Farmasko, Ukraine). The condition of vaginal biocenosis was assessed according to «Femoflor-Screen», polymerase chain reaction in real time. The formation of biofilms was explored with the study of the ability of pure culture Gardnerellaе to adhesion on the surface of microplates and the addition of crystal violet indicator. Optical density was measured with the photometer «Multiskan EX-355» (Labsystems, China). These vaginal micribiota of the patients in control group were inconsistent with microbiocenosis of I and II type (normocenosis, intermediate type). II type of the vagina normocenosis was found in 29,4 % patients with long usage of contraception up to 12 month. III type of vaginal microbiocenosis was observed in 55, 8 % of examined patients within the duration of IUD use from 2 till 5 years. Microbiocenosis of women with IV type of smear was inconsistent with non-specific or hybrid vaginitis (IUD duration more than 5 years). Changes of total bacterial exudates, detection of opportunistic or pathogenic flora, especially for mixed bacterial associations for women with intra-uterine devices gives rise to vaginal biofilm formation. The type of vaginal biocenosis depends on the duration of IUC use. рН level of vaginal secretion, which conforms to the quantitative evaluation of microbiocenosis and the availability of lactobacteria in it can be used as an indicator for condition of vaginal biocenosis. pН level of vaginal secretion can be a screen control of vaginal biocenosis condition during intrauterine devices stay for the control of its durability in the uterus.