Kuntai Capsule Improves Ovarian Function in Rats with Premature Ovarian Failure After Transplantation of Cryopreserved Ovarian Tissue by Regulating Sex Hormones and Apoptosis.

OBJECTIVE

Our study aimed to investigate the therapeutic effects of the Kuntai capsule in improving ovarian function in rats with transplantation of cryopreserved ovary.

METHODS

Two mice ovary cell lines were cultured with Kuntai capsule decoction, and cell apoptosis was detected by MTT assay. A total of 90 SPF Sprague Dawley rats were included in this study. Thirty rats were used as the control group (group A), and not treated with any surgical operation. The remaining 60 rats were subjected to surgery to collect ovarian tissues and to construct a premature ovarian failure model. Ovarian tissues were cryopreserved, thawed, and transplanted back to ovaries. Sixty rats with ovary transplantation were randomly divided into group B and group C. Rats in group B were treated with Kuntai capsule at a dose of 0.1 capsule per day while rats in group C were fed with normal food. Serum levels of estradiol (E2) and follicular stimulating hormone (FSH) were detected. Expressions of several cytokines, including hepatocyte growth factor (HGF), vascular endothelial growth factor (VEGF), insulin-like growth factor 1 (IGF-1), and apoptotic factors caspase-3 and p53, were also detected.

RESULTS

Kuntai capsule decoction inhibited apoptosis of in vitro cultured mice ovary cells. Furthermore, the Kuntai capsule promoted the recovery of E2 and FSH to normal levels and regulated the abnormal expression of HGF, VEGF, and IGF-1 and apoptotic factors caspase-3 and p53 in rats with premature ovarian failure after homotopic transplantation of ovarian tissue.

CONCLUSION

The Kuntai capsule can improve ovarian functions by regulating sexual hormones and cell apoptosis in rats with premature ovarian failure after homotopic transplantation of cryopreserved ovary tissue.

KEYWORDS: premature ovarian failure, Kuntai capsule, apoptosis, sexual hormones, homotopic transplantation of cryopreserved ovary tissue.