[Identification of metastasis-associated proteins of ovarian cancer by proteomics].

OBJECTIVE

To investigate the differenal protein expression profiles of ovarian tumor cell lines with distinct metastatic abilities.

METHODS

The ovarian cancer cell line HO8910 and HO8910pm, derived from same parental cells but exhibited different metastatic ability, were investigated by two-dimensional gel electrophoresis (2-DE)-MALDI-TOF-MS proteomic approach.

RESULTS

Thirty-nine proteins were detected by 2-DE to have expression disparity levels over 2 folds between two cell lines. Eighteen of them were identified by MALDI-TOF-MS. The proteins are involved in apoptosis, extra cellular matrix (ECM), cytoskeleton, growth factor, glycolysis, protein metabolism and immune system.

CONCLUSION

The data are valuable for the identification of differentially expressed proteins involved in the biological behavior of human ovarian cancer.