[Inhibitory effect of breast cancer metastasis suppressor l gene on metastasis of human ovarian cancer cell in vitro and in vivo].

OBJECTIVE

To study the inhibition effects of breast cancer metastasis suppressor l (BRMS1) gene on metastasis of human ovarian cancer cell in vivo and in vitro.

METHODS

BRMS1 gene was transfected into human ovarian cancer cell line A2780 by liposome transfection method. The cells were divided into 3 groups: transfected group with pcDNA3-BRMS1, negative control group with empty plasmid pcDNA3, blank control group without any transfection. Proliferation, apoptosis, growth rate, capability of colony formation, gap junctional intercellular communication (GJIC), capability of adhesion, changes of surface and internal structures of cells were observed in vitro. The cells were injected into athymic mice to establish animal tumor models, and inhibition of the tumorigenicity and metastasis were observed in vivo.

RESULTS

BRMS1 gene was transfected into A2780 cell line successfully. There were no significant differences in the growth rate among transfected group, negative control group and blank control group (P > 0.05). The amounts of cell clones per well were 40 +/- 4 in transfected group, 42 +/- 7 in blank control group and 39 +/- 4 in negative control group (P > 0.05 between each two groups). The ratios of S vs G(2)/M and G(0)/G(1) were not significantly different in three groups (P > 0.05). The ultramicrostructure of cells detected by electron microscope showed that GJIC function in transfected group was higher than that in the other two groups. While in migration assay, the numbers of cells in lower chamber passing through the membrane in transfected group, blank control group and negative control group were 112 +/- 23, 306 +/- 49 and 322 +/- 91, respectively; with significant differences among 3 groups (P < 0.01).

CONCLUSION

BRMS1 gene could suppress metastasis of ovarian cancer in vitro and in vivo.