Establishment of long-term feline oviduct epithelial spheroids in vitro culture system.

This study aimed to characterize a long-term in vitro culture of feline oviduct epithelial spheroids (fOES) and to compare the effects of supplementation with fetal bovine serum (FBS) and bovine serum albumin (BSA) on fOES parameters. BSA improved structural preservation, reduced oxidative stress, and enhanced cell viability and motility. Despite morphological and metabolic changes, fOES preserved functional characteristics, supporting their use as a reliable in vitro model for reproductive studies in felids.