Journal of traditional Chinese medicine = Chung i tsa chih ying wen pan · 2016
Effects of electronically stimulating Tianshu (ST 25) and Dachangshu (BL 25), Quchi (LI 11) and Shangjuxu (ST 37) on the expressions of jejunum c-kit protein and c-kit mRNA in rats with functional diarrhea.
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Wang Yuan, Niu Wenmin, Yang Xiaohang, Liu Na, Liu Zhibin
Аннотация
OBJECTIVE: To investigate the effects of electronically stimulating Tianshu (ST 25) and Dachangshu (BL 25), Quchi (LI 11) and Shangjuxu (ST 37) on the jejunum c-kit protein and c-kit mRNA in rats with functional diarrhea (FD).
METHODS: FD models were established through intragastric administration with folium sennae. Experimental rats were then divided into 4 groups: blank group, model group, electroacupuncture group Ⅰ [Tianshu (ST 25) and Dachangshu (BL 25) of both sides] and electroacupuncture group Ⅱ [Quchi (Li 11) and Shangjuxu (ST 37) of both sides], 10 in each. After treatment with electroacupuncture for 10 days, The expressions of jejunum c-kit protein and c-kit mRNA in each group were detected with Western blot and Real-Time quantitative real-time polymerase chain reaction (PCR).
RESULTS: The expressions of c-kit protein and c-kit mRNA in the model group increased significantly compared to those in the blank group (P < 0.01); the expressions in electroacupuncture group Ⅰsignificantly decreased compared to those in the model group (P < 0.01).
CONCLUSION: Our findings suggest that electronicall stimulating both Tianshu (ST 25) and Dachangshu (BL 25) significantly increased the expressions of jejunum c-kit protein and c-kit mRNA in FD rats, which means the treatment might have better therapeutic effects on FD.
METHODS: FD models were established through intragastric administration with folium sennae. Experimental rats were then divided into 4 groups: blank group, model group, electroacupuncture group Ⅰ [Tianshu (ST 25) and Dachangshu (BL 25) of both sides] and electroacupuncture group Ⅱ [Quchi (Li 11) and Shangjuxu (ST 37) of both sides], 10 in each. After treatment with electroacupuncture for 10 days, The expressions of jejunum c-kit protein and c-kit mRNA in each group were detected with Western blot and Real-Time quantitative real-time polymerase chain reaction (PCR).
RESULTS: The expressions of c-kit protein and c-kit mRNA in the model group increased significantly compared to those in the blank group (P < 0.01); the expressions in electroacupuncture group Ⅰsignificantly decreased compared to those in the model group (P < 0.01).
CONCLUSION: Our findings suggest that electronicall stimulating both Tianshu (ST 25) and Dachangshu (BL 25) significantly increased the expressions of jejunum c-kit protein and c-kit mRNA in FD rats, which means the treatment might have better therapeutic effects on FD.