Effects of electronically stimulating Tianshu (ST 25) and Dachangshu (BL 25), Quchi (LI 11) and Shangjuxu (ST 37) on the expressions of jejunum c-kit protein and c-kit mRNA in rats with functional diarrhea.

OBJECTIVE

To investigate the effects of electronically stimulating Tianshu (ST 25) and Dachangshu (BL 25), Quchi (LI 11) and Shangjuxu (ST 37) on the jejunum c-kit protein and c-kit mRNA in rats with functional diarrhea (FD).

METHODS

FD models were established through intragastric administration with folium sennae. Experimental rats were then divided into 4 groups: blank group, model group, electroacupuncture group Ⅰ [Tianshu (ST 25) and Dachangshu (BL 25) of both sides] and electroacupuncture group Ⅱ [Quchi (Li 11) and Shangjuxu (ST 37) of both sides], 10 in each. After treatment with electroacupuncture for 10 days, The expressions of jejunum c-kit protein and c-kit mRNA in each group were detected with Western blot and Real-Time quantitative real-time polymerase chain reaction (PCR).

RESULTS

The expressions of c-kit protein and c-kit mRNA in the model group increased significantly compared to those in the blank group (P < 0.01); the expressions in electroacupuncture group Ⅰsignificantly decreased compared to those in the model group (P < 0.01).

CONCLUSION

Our findings suggest that electronicall stimulating both Tianshu (ST 25) and Dachangshu (BL 25) significantly increased the expressions of jejunum c-kit protein and c-kit mRNA in FD rats, which means the treatment might have better therapeutic effects on FD.