Zhonghua yi xue za zhi · 1996
[Reconstitution of Hu-SCID mice transplanted with human tonsillar lymphocytes immunized with dengue virus-2 in vitro and its immune response].
Аннотация на английскомСтатьяПоддержка исследования: вне правительства СШАНазвание и аннотация: Перевод пока не подготовлен
Обработка статьи · этапы, ошибки и управление
Загрузка состояния…
Ошибки и ограничения
История · было → стало
Состояние обновляется каждые 5 секунд, пока блок открыт. Процент показывается только там, где известен объём работы. Пауза останавливает продолжение этапа; текущий запрос к источнику может завершиться.
Zuo L, Guo H, Zhang C
Аннотация
OBJECTIVE: To study the measure for the reconstitution of severe combined immunodeficient disease mice (SCID mice) transplanted with human lymphocytes immunized with specific antigen in vitro.
METHODS: Hu-SCID mice were constructed by intraperitoneal (i.p.) transfer of human tonsillar lymphocytes (TLC) first immunized with dengue virus-2 (DV2) in vitro, then boostered with different doses of DV2 antigen in vivo.
RESULTS: Human immunoglobulin (Ig) and specific human anti-DV2 antibodies could be detected in sera of the hu-SCID mice by ELISA. Two weeks after transplantion of the in vitro-immunized hu-TLC, the highest human IgG concentration was 412.86 micrograms/ml, two or four weeks later specific human anti-DV2 IgM or IgG and the neutralizing antibody could found in these sera, respectively, the highest titer was 1:6400. The levels of human IgG and human anti-DV2 antibodies were related to the dose of DV2 antigen. The presence of human B cells (CD20+) and T/cells (CD3+, CD4+, CD8+) were shown in the sleen, lung, intestine and mesentery of the hu-SCID mice as determined by immunoperoxidase staining, and the cells were distributed correspondingly with the lymphocyte cells in proper human tissue. Human anti-DV2 IgG could also be detected in the spleen cell culture obtained from the SCID mice.
CONCLUSION: By co-immunized in vitro and in vivo the SCID mice deduced stronger immune response for specific antigen, and the reconstitution of the human lymphocytes could be established successfully.
METHODS: Hu-SCID mice were constructed by intraperitoneal (i.p.) transfer of human tonsillar lymphocytes (TLC) first immunized with dengue virus-2 (DV2) in vitro, then boostered with different doses of DV2 antigen in vivo.
RESULTS: Human immunoglobulin (Ig) and specific human anti-DV2 antibodies could be detected in sera of the hu-SCID mice by ELISA. Two weeks after transplantion of the in vitro-immunized hu-TLC, the highest human IgG concentration was 412.86 micrograms/ml, two or four weeks later specific human anti-DV2 IgM or IgG and the neutralizing antibody could found in these sera, respectively, the highest titer was 1:6400. The levels of human IgG and human anti-DV2 antibodies were related to the dose of DV2 antigen. The presence of human B cells (CD20+) and T/cells (CD3+, CD4+, CD8+) were shown in the sleen, lung, intestine and mesentery of the hu-SCID mice as determined by immunoperoxidase staining, and the cells were distributed correspondingly with the lymphocyte cells in proper human tissue. Human anti-DV2 IgG could also be detected in the spleen cell culture obtained from the SCID mice.
CONCLUSION: By co-immunized in vitro and in vivo the SCID mice deduced stronger immune response for specific antigen, and the reconstitution of the human lymphocytes could be established successfully.