Acta tropica · 2026
A rapid visual detection method for Schistosoma japonicum in Oncomelania hupensis snails using recombinase-aided amplification and lateral flow dipsticks.
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Ye Yuying, Liu Yanhong, Bi Niannian, Xiong Chunrong, Zhang Jianfeng, Zhao Song, Yang Kun
Аннотация
BACKGROUND: Effective surveillance of infected Oncomelania hupensis snails is pivotal for schistosomiasis elimination, yet traditional methods lack sensitivity and molecular assays require sophisticated equipment.
METHODS: We developed an RAA-LFD method targeting the SjG28 gene, using biotin-labeled primers and FAM-labeled probes, with isothermal amplification at 39°C for 15 min and visual readout on dipsticks.
RESULTS: The assay achieved a detection limit of 1 pg/μL genomic DNA and 10 copies/μL plasmid DNA, with no cross-reactivity against six other parasites. It detected as few as one infected snail among 50 and identified infections from day 7 post-infection.
CONCLUSION: This equipment-free, visual RAA-LFD method offers a practical solution for field-based surveillance, with potential to strengthen schistosomiasis elimination efforts in resource-limited settings.
METHODS: We developed an RAA-LFD method targeting the SjG28 gene, using biotin-labeled primers and FAM-labeled probes, with isothermal amplification at 39°C for 15 min and visual readout on dipsticks.
RESULTS: The assay achieved a detection limit of 1 pg/μL genomic DNA and 10 copies/μL plasmid DNA, with no cross-reactivity against six other parasites. It detected as few as one infected snail among 50 and identified infections from day 7 post-infection.
CONCLUSION: This equipment-free, visual RAA-LFD method offers a practical solution for field-based surveillance, with potential to strengthen schistosomiasis elimination efforts in resource-limited settings.