Journal of ethnopharmacology · 2026
Buxue Yimu Pills alleviate uterine injury after incomplete abortion by regulating OXTR-mediated PI3K/AKT and JAK2/STAT3 signaling pathways.
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Feng Mengjiao, Chen Qingshan, Li Hao, Gong Huan, Wang Liang, Qian Yong, Zhang Suying, Zhang Hai
Аннотация
ETHNOPHARMACOLOGICAL RELEVANCE: Buxue Yimu Pills (BYP) is a well-established traditional Chinese medicine widely used for postpartum recovery. However, its bioactive constituents and mechanisms of action in the treatment of incomplete abortion-induced uterine injury remain unclear.
OBJECTIVE: This study aimed to identify the candidate bioactive constituents and molecular targets of BYP and elucidate the synergistic mechanisms underlying its therapeutic effects.
METHODS: Incomplete abortion was established in rats by co-administration of mifepristone and misoprostol, and the effects of BYP were assessed by ultrasonographic, histopathological and biochemical analyses. An integrated analytical strategy incorporating serum pharmacochemistry, network pharmacology, transcriptomics, molecular dynamics simulation and surface plasmon resonance (SPR) was then used to identify bioactive constituents and investigate the molecular mechanisms of BYP.
RESULTS: A total of 82 constituents were identified in BYP, and 104 absorbed constituents were detected in rat serum, including 47 prototype constituents and 57 metabolites. BYP significantly alleviated uterine injury, reduced inflammatory responses and collagen deposition, and promoted endometrial repair. Furthermore, integrated analyses indicated that BYP may coordinate the 3R processes, which consist of residual tissue removal, inflammatory microenvironment remodeling, and tissue repair, through regulating the OXTR, JAK2/STAT3, and PI3K/AKT signaling pathways. OXTR inhibition weakened BYP-induced activation of PI3K/AKT and partially reversed the inhibition of JAK2/STAT3 signaling. Moreover, molecular dynamics simulations and SPR analyses supported stable and direct interactions of leonurine with OXTR, ferulic acid with AKT, and astragaloside IV with STAT3.
CONCLUSIONS: BYP alleviates uterine injury after incomplete abortion through OXTR-mediated PI3K/AKT and JAK2/STAT3 signaling pathways.
OBJECTIVE: This study aimed to identify the candidate bioactive constituents and molecular targets of BYP and elucidate the synergistic mechanisms underlying its therapeutic effects.
METHODS: Incomplete abortion was established in rats by co-administration of mifepristone and misoprostol, and the effects of BYP were assessed by ultrasonographic, histopathological and biochemical analyses. An integrated analytical strategy incorporating serum pharmacochemistry, network pharmacology, transcriptomics, molecular dynamics simulation and surface plasmon resonance (SPR) was then used to identify bioactive constituents and investigate the molecular mechanisms of BYP.
RESULTS: A total of 82 constituents were identified in BYP, and 104 absorbed constituents were detected in rat serum, including 47 prototype constituents and 57 metabolites. BYP significantly alleviated uterine injury, reduced inflammatory responses and collagen deposition, and promoted endometrial repair. Furthermore, integrated analyses indicated that BYP may coordinate the 3R processes, which consist of residual tissue removal, inflammatory microenvironment remodeling, and tissue repair, through regulating the OXTR, JAK2/STAT3, and PI3K/AKT signaling pathways. OXTR inhibition weakened BYP-induced activation of PI3K/AKT and partially reversed the inhibition of JAK2/STAT3 signaling. Moreover, molecular dynamics simulations and SPR analyses supported stable and direct interactions of leonurine with OXTR, ferulic acid with AKT, and astragaloside IV with STAT3.
CONCLUSIONS: BYP alleviates uterine injury after incomplete abortion through OXTR-mediated PI3K/AKT and JAK2/STAT3 signaling pathways.