Eye (London, England) · 2026
Identification of a translatable rabbit model for corneal endothelial dysfunction.
СтатьяНазвание и аннотация: Перевод пока не подготовлен
Обработка статьи · этапы, ошибки и управление
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История · было → стало
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Ortega-Llamas Laura, Pérez-Perdomo Mayelín, Blanco-Blanco Mario, Villalba-González Marta, González-López Ana, Alba-Molina David, Cantarero Irene, Jiménez-Gómez Yolanda, González-Andrades Miguel
Аннотация
PURPOSE: To explore the potential of an adult rabbit descemetorhexis model to recapitulate the damage to the endothelium and Descemet's membrane (DM) seen in humans.
METHODS: Five corneas of 5 female New Zealand rabbits aged 24-26 months underwent 5-mm descemetorhexis; five unwounded corneas from 5 rabbits served as controls. Animals were followed up for 12-week and post-surgical clinical evaluation was performed using slit-lamp, macro images, and optical coherence tomography (OCT). Histological and immunohistochemical analyses were performed post-sacrifice.
RESULTS: Corneal transparency analysed by slit-lamp demonstrated that 2 weeks after descemetorhexis, all adult rabbits displayed grade 3 corneal opacity that was reduced to grade 2-3 and remained at this level until the study endpoint. Corroborating these results, quantitative analysis of macro images showed a significant reduction in corneal transparency during the entire follow-up period, with remarkable effect at 2 weeks (P < 0.05 vs. pre-surgery). Besides, OCT results showed a significant increase in corneal and stromal thickness and fibrosis at 4/8-week post-surgery (P < 0.05 vs. pre-surgery). By 12 weeks, rabbits maintained remarkable fibrosis (P = 0.013 vs. pre-surgery). Finally, histological and immunohistochemical analyses showed that the descemetorhexis group displayed absence of the DM-endothelium complex in the surgical center, which was associated with non-expression of Na+/K+-ATPase and Zonula occludens-1. An altered collagen matrix with myofibroblasts in the posterior stroma was also found in the surgical center compared with the other analyzed regions in both groups.
CONCLUSIONS: Our results indicate that descemetorhexis in adult rabbits could be a translatable corneal endothelial dysfunction (CED) model for studying the safety and efficacy of treatments for CED.
METHODS: Five corneas of 5 female New Zealand rabbits aged 24-26 months underwent 5-mm descemetorhexis; five unwounded corneas from 5 rabbits served as controls. Animals were followed up for 12-week and post-surgical clinical evaluation was performed using slit-lamp, macro images, and optical coherence tomography (OCT). Histological and immunohistochemical analyses were performed post-sacrifice.
RESULTS: Corneal transparency analysed by slit-lamp demonstrated that 2 weeks after descemetorhexis, all adult rabbits displayed grade 3 corneal opacity that was reduced to grade 2-3 and remained at this level until the study endpoint. Corroborating these results, quantitative analysis of macro images showed a significant reduction in corneal transparency during the entire follow-up period, with remarkable effect at 2 weeks (P < 0.05 vs. pre-surgery). Besides, OCT results showed a significant increase in corneal and stromal thickness and fibrosis at 4/8-week post-surgery (P < 0.05 vs. pre-surgery). By 12 weeks, rabbits maintained remarkable fibrosis (P = 0.013 vs. pre-surgery). Finally, histological and immunohistochemical analyses showed that the descemetorhexis group displayed absence of the DM-endothelium complex in the surgical center, which was associated with non-expression of Na+/K+-ATPase and Zonula occludens-1. An altered collagen matrix with myofibroblasts in the posterior stroma was also found in the surgical center compared with the other analyzed regions in both groups.
CONCLUSIONS: Our results indicate that descemetorhexis in adult rabbits could be a translatable corneal endothelial dysfunction (CED) model for studying the safety and efficacy of treatments for CED.