Analytica chimica acta · 2026
A Lab-in-Tube platform based on Ag+-coupled hairpin aptamer and gold nanoparticles-immobilized DNA hydrogel for portable and ultrasensitive biosensing.
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Liu Zhaoying, Kong Yuan, Yang Kan, Chen Guangyun, Li Ming
Аннотация
BACKGROUND: The overuse of ampicillin (AMP), a pivotal antibiotic, poses severe environmental and ecological risks, necessitating portable and precise monitoring.
RESULTS: A highly integrated Lab-in-Tube biosensing platform based on Ag+-coupled hairpin (Ag@hairpin) aptamer and gold nanoparticles-immobilized DNA (AuNP@DNA) hydrogel for the portable and ultrasensitive detection of AMP was developed. In this pre-assembled tube platform, Ag@hairpin aptamers and AuNP@DNA hydrogels were immobilized on the inner bottom and cap of the tube, respectively. Upon introduction of AMP, the released Ag+ triggered Ag-DNAzyme to cleave S-DNA, generating abundant AuNP. These AuNP were subsequently introduced into a glucose/FeNi-doped polydopamine nanozyme (FeNi@PDA) system to generate ultrasensitive colorimetric signals. The biosensing requires only a simple two-step upside down operation in the sealed tube, achieving an excellent limit of detection (LOD) of 1.02 pg/mL. The platform demonstrated high specificity (cross-reactivity less than 4.31%) and practical applicability in environmental samples. Furthermore, the advantages of miniaturized platform, simplified operation and portability had demonstrated.
SIGNIFICANCE: By integrating accurate biosensing with an ultrasensitive enzyme cascade system, this Lab-in-Tube platform may offer an advanced strategy for the portable and on-site detection of other targets.
RESULTS: A highly integrated Lab-in-Tube biosensing platform based on Ag+-coupled hairpin (Ag@hairpin) aptamer and gold nanoparticles-immobilized DNA (AuNP@DNA) hydrogel for the portable and ultrasensitive detection of AMP was developed. In this pre-assembled tube platform, Ag@hairpin aptamers and AuNP@DNA hydrogels were immobilized on the inner bottom and cap of the tube, respectively. Upon introduction of AMP, the released Ag+ triggered Ag-DNAzyme to cleave S-DNA, generating abundant AuNP. These AuNP were subsequently introduced into a glucose/FeNi-doped polydopamine nanozyme (FeNi@PDA) system to generate ultrasensitive colorimetric signals. The biosensing requires only a simple two-step upside down operation in the sealed tube, achieving an excellent limit of detection (LOD) of 1.02 pg/mL. The platform demonstrated high specificity (cross-reactivity less than 4.31%) and practical applicability in environmental samples. Furthermore, the advantages of miniaturized platform, simplified operation and portability had demonstrated.
SIGNIFICANCE: By integrating accurate biosensing with an ultrasensitive enzyme cascade system, this Lab-in-Tube platform may offer an advanced strategy for the portable and on-site detection of other targets.