Frontiers in plant science · 2026
Exogenous 24-epibrassinolide enhances chilling tolerance in Phalaenopsis-type Dendrobium through coordinated physiological and transcriptomic responses.
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Yu Xiaoyun, Wei Xinjie, Yao Tongyan, Luo Xiaoyan, Yi Shuangshuang, Liao Yi, Li Chonghui, Lu Shunjiao
Аннотация
INTRODUCTION: Phalaenopsis-type Dendrobium is a commercially important tropical orchid that is highly sensitive to chilling, which can cause premature leaf senescence and abscission.
METHODS: We evaluated the protective effect of exogenous 24-epibrassinolide (EBR) and integrated time-resolved RNA-seq with physiological traits previously characterized in the same experimental framework. A concentration of 2.0 mg/L was selected for transcriptomic analysis based on the refined dose-response experiment. WGCNA was performed using 18 EBR-treated libraries. Global GO and KEGG outputs were retained as descriptive overviews, whereas quantitative functional inference was based on module-specific analyses with complete multiple-testing-adjusted statistics.
RESULTS: EBR delayed leaf yellowing and abscission. Differential-expression analysis revealed time-dependent transcriptional responses during chilling and recovery. WGCNA identified trait-associated modules with contrasting temporal relationships to physiological traits: the pink module was positively associated with MDA and proline and negatively associated with chlorophyll, whereas the antiquewhite4 (AW4) module showed the opposite pattern. Pink-module enrichment emphasized central carbon and energy metabolism, lipid turnover, peroxisomal processes, autophagy, and cellular transport/homeostasis. AW4 GO enrichment was dominated by transmembrane and metabolite transport; no AW4 KEGG pathway remained significant after multiple-testing correction.
DISCUSSION: These results support an association between EBR treatment and coordinated metabolic, membrane-homeostasis, and stress-response adjustments during chilling and recovery in Phalaenopsis-type Dendrobium.
METHODS: We evaluated the protective effect of exogenous 24-epibrassinolide (EBR) and integrated time-resolved RNA-seq with physiological traits previously characterized in the same experimental framework. A concentration of 2.0 mg/L was selected for transcriptomic analysis based on the refined dose-response experiment. WGCNA was performed using 18 EBR-treated libraries. Global GO and KEGG outputs were retained as descriptive overviews, whereas quantitative functional inference was based on module-specific analyses with complete multiple-testing-adjusted statistics.
RESULTS: EBR delayed leaf yellowing and abscission. Differential-expression analysis revealed time-dependent transcriptional responses during chilling and recovery. WGCNA identified trait-associated modules with contrasting temporal relationships to physiological traits: the pink module was positively associated with MDA and proline and negatively associated with chlorophyll, whereas the antiquewhite4 (AW4) module showed the opposite pattern. Pink-module enrichment emphasized central carbon and energy metabolism, lipid turnover, peroxisomal processes, autophagy, and cellular transport/homeostasis. AW4 GO enrichment was dominated by transmembrane and metabolite transport; no AW4 KEGG pathway remained significant after multiple-testing correction.
DISCUSSION: These results support an association between EBR treatment and coordinated metabolic, membrane-homeostasis, and stress-response adjustments during chilling and recovery in Phalaenopsis-type Dendrobium.