Development and validation of an environmentally friendly spectrofluorimetric method for dimethindene maleate determination: Application to dosage forms and biological fluids.

A novel, ecologically friendly, and thoroughly investigated fluorometric approach was established to measure dimethindene maleate (DMD), an antihistaminic medication intended to diminish capillary hyperpermeability during early allergic responses. The approach includes monitoring DMD fluorescence intensity in an aqueous solution. The technique was validated in compliance with ICH standards. A correlation coefficient of 0.9998 was found between DMD concentration and fluorescence intensity for concentrations ranging from 100 to 2000 ng mL-1. The limit of detection and limit of quantification were determined to be 26.7 and 80.9 ng mL-1, respectively. This technique could be used to quantify DMD in its pure form and to investigate medicinal formulations. Additionally, it was utilized to assess DMD concentrations in spiked human plasma. The greenness of the proposed technique was validated by operating several green analytical criteria.