Analytical and quantitative cytology and histology · 2010
DNA damage in Mexican women with cervical dysplasia evaluated by comet assay.
Клиническое исследованиеСтатьяНазвание и аннотация: Перевод пока не подготовлен
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Cortés-Gutiérrez Elva I, Dávila-Rodríguez Martha I, Zamudio-González Estela A, Aguado-Barrera Miguel E, Vargas-Villarreal Javier, Cerda-Flores Ricardo M
Аннотация
OBJECTIVE: To investigate the association between the progressive stage of cervical dysplasia and DNA damage by comet assay.
STUDY DESIGN: A hospital-based, unmatched, case-control study was performed. DNA damage levels (none, low, medium and high) in the cervical epithelial cells of 31 women (10 with low grade squamous intraepithelial lesions [LSIL], 10 with high grade [HSIL] and 11 with no cervical lesion) were evaluated using the comet assay.
RESULTS: A significant increase in medium DNA damage was observed in women with HSIL (17 +/- 8.9) relative to that in the control women (9 +/- 6.1). A significant increase in high DNA damage was also observed in women with LSIL (23 +/- 15.4) or HSIL (32 +/- 13.1) relative to that in the control women (12 +/- 7.9).
CONCLUSION: These findings confirm that the grade of a cervical lesion correlates with the degree of genomic instability.
STUDY DESIGN: A hospital-based, unmatched, case-control study was performed. DNA damage levels (none, low, medium and high) in the cervical epithelial cells of 31 women (10 with low grade squamous intraepithelial lesions [LSIL], 10 with high grade [HSIL] and 11 with no cervical lesion) were evaluated using the comet assay.
RESULTS: A significant increase in medium DNA damage was observed in women with HSIL (17 +/- 8.9) relative to that in the control women (9 +/- 6.1). A significant increase in high DNA damage was also observed in women with LSIL (23 +/- 15.4) or HSIL (32 +/- 13.1) relative to that in the control women (12 +/- 7.9).
CONCLUSION: These findings confirm that the grade of a cervical lesion correlates with the degree of genomic instability.