Burns & trauma · 2026
Cryogenic 3D-printed PLGA/nano-selenium scaffold for bone regeneration: a dual-functional strategy synergistically regulating macrophage polarization and osteogenic differentiation.
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Cheng Shengwen, Wang Yuqiao, Zhai Yu, Dong Mingfei, Peng Xiaole, Luo Xiaohong, Liu Jiacheng, Liu Junyan, He Yi, Li Xiaoxiong, Huang Ke, Liu Senrui, Liu Fengyuan, Zhang Yuwei, Li Zhong Alan, Huang Wei, Zhao Chen, Lei Yiting
Аннотация
BACKGROUND: Autologous and allogeneic bone grafts are primarily used for bone tissue defects; however, they have limitations such as limited supply, donor site morbidity, and immune rejection risks. Therefore, substitute synthetic bone grafts are required. This study aims to develop a cryogenic 3D-printed PLGA/HA@SeNPs scaffold that synergistically regulates macrophage polarization and osteogenic differentiation for bone regeneration.
METHODS: Using low-temperature 3D printing combined with freeze-drying technology, a hierarchically porous PLGA/HA@SeNPs composite scaffold was fabricated by compositing poly(lactic-co-glycolic acid) (PLGA) with hyaluronic acid-modified selenium (Se) nanoparticles (HA@SeNPs), enabling sustained immunomodulation and osteogenic activity through its engineered microtopography and bioactive components.
RESULTS: In vitro evaluations confirmed that the unique microstructure and sustained Se release from HA@SeNPs synergistically promoted macrophage polarization toward the M2 phenotype, accompanied by enhanced osteogenic differentiation as shown by upregulation of Runx2 and osteocalcin and accelerated matrix mineralization. Implantation into a rat femoral critical-sized defect model resulted in substantially improved bone repair and architectural restoration.
CONCLUSIONS: These findings indicate that the intrinsic physicochemical properties of the PLGA/HA@SeNPs scaffold orchestrate a favorable osteo-immune environment, positioning it as a promising platform for bone regeneration.
METHODS: Using low-temperature 3D printing combined with freeze-drying technology, a hierarchically porous PLGA/HA@SeNPs composite scaffold was fabricated by compositing poly(lactic-co-glycolic acid) (PLGA) with hyaluronic acid-modified selenium (Se) nanoparticles (HA@SeNPs), enabling sustained immunomodulation and osteogenic activity through its engineered microtopography and bioactive components.
RESULTS: In vitro evaluations confirmed that the unique microstructure and sustained Se release from HA@SeNPs synergistically promoted macrophage polarization toward the M2 phenotype, accompanied by enhanced osteogenic differentiation as shown by upregulation of Runx2 and osteocalcin and accelerated matrix mineralization. Implantation into a rat femoral critical-sized defect model resulted in substantially improved bone repair and architectural restoration.
CONCLUSIONS: These findings indicate that the intrinsic physicochemical properties of the PLGA/HA@SeNPs scaffold orchestrate a favorable osteo-immune environment, positioning it as a promising platform for bone regeneration.