Journal of clinical immunology · 2009
CD3zeta expression and T cell proliferation are inhibited by TGF-beta1 and IL-10 in cervical cancer patients.
СтатьяПоддержка исследования: вне правительства СШАНазвание и аннотация: Перевод пока не подготовлен
Обработка статьи · этапы, ошибки и управление
Загрузка состояния…
Ошибки и ограничения
История · было → стало
Состояние обновляется каждые 5 секунд, пока блок открыт. Процент показывается только там, где известен объём работы. Пауза останавливает продолжение этапа; текущий запрос к источнику может завершиться.
Díaz-Benítez Cinthya E, Navarro-Fuentes Karla R, Flores-Sosa Jacqueline A, Juárez-Díaz Janet, Uribe-Salas Felipe J, Román-Basaure Edgar, González-Mena Ludwig E, Alonso de Ruíz Patricia, López-Estrada Guillermina, Lagunas-Martínez Alfredo, Bermúdez-Morales Victor H, Alcocer-González Juan M, Martínez-Barnetche Jesús, Hernández-Pando Rogelio, Rosenstein Yvonne, Moreno José, Madrid-Marina Vicente
Аннотация
INTRODUCTION: Cervical cancer development from a squamous intraepithelial lesion is thought to be favored by an impaired T cell immunity. We evaluated parameters of T cell alterations such as proliferation, cytokine, and CD3zeta expression in peripheral blood and tumor-infiltrating T lymphocytes from women with squamous intraepithelial lesions (SIL) or cervical cancer (CC).
RESULTS AND DISCUSSION: T cell proliferation and cytokine messenger RNA (mRNA) expression were similar in women with SIL and healthy donors, whereas low T cell proliferation and lower mRNA expression of IL-2, IL-10 and IFN-gamma were observed in women with CC. Moreover, infiltrating cells showed marginal responses. We also found that CD3zeta mRNA expression, whose protein is required for T cell activation, correlated with a decreased proliferation in advanced stages of the disease.
CONCLUSION: Experiments with T cells from healthy donors in the presence TGF-beta1 or IL-10 suggest that these cytokines have a relevant role in T cell responses during CC progression.
RESULTS AND DISCUSSION: T cell proliferation and cytokine messenger RNA (mRNA) expression were similar in women with SIL and healthy donors, whereas low T cell proliferation and lower mRNA expression of IL-2, IL-10 and IFN-gamma were observed in women with CC. Moreover, infiltrating cells showed marginal responses. We also found that CD3zeta mRNA expression, whose protein is required for T cell activation, correlated with a decreased proliferation in advanced stages of the disease.
CONCLUSION: Experiments with T cells from healthy donors in the presence TGF-beta1 or IL-10 suggest that these cytokines have a relevant role in T cell responses during CC progression.
Предметные рубрики MeSH
AdultAgedCD3 ComplexCell Line, TumorCell ProliferationCulture Media, ConditionedFemaleHeLa CellsHumansInterferon-gammaInterleukin-10Interleukin-2Lymphocytes, Tumor-InfiltratingMiddle AgedPhytohemagglutininsT-LymphocytesTransforming Growth Factor beta1Uterine Cervical NeoplasmsUterine Cervical Dysplasia