Frontiers in nutrition · 2026
Structural characterization and in vitro myocardial injury protection potential of one acidic polysaccharide from blueberry (Vaccinium spp.) pomace.
СтатьяНазвание и аннотация: Перевод пока не подготовлен
Обработка статьи · этапы, ошибки и управление
Загрузка состояния…
Ошибки и ограничения
История · было → стало
Состояние обновляется каждые 5 секунд, пока блок открыт. Процент показывается только там, где известен объём работы. Пауза останавливает продолжение этапа; текущий запрос к источнику может завершиться.
Zhang Beibei, Zhang Qiwen, Huang Ning, Jia Mengmeng, Xing Han, Yang Yongjie, Xue Dinglei, Yang Jing, Qin Zifei
Аннотация
INTRODUCTION: Blueberry pomace is the by-product of blueberry (Vaccinium spp.) fruits. This study aimed to prepare blueberry pomace polysaccharide (BBPP) and explore its myocardial injury protection potential.
METHODS: An acidic polysaccharide (BBPP80-1a) was isolated and purified from active BBPP80-1 fraction. Its structure was characterized based on some approaches. Its myocardial injury protection potential was performed in hypoxia/reoxygenation (H/R)-induced H9c2 cells injury model.
RESULTS: The molecular weight of BBPP80-1a was 164.91 kDa. The ratio of galacturonic acid (GalA) was 50.92% in methylation results. Further, nuclear magnetic resonance data suggested repetitive → 4)-α-D-GalpA-(1 → and → 4)-α-D-GalpA-6-OMe-(1 → residues occupied the homogalacturonan region of backbone. It had a highly porous, reticular, and heterogeneous microstructures. Moreover, BBPP80-1a can obviously protect H9c2 cells from H/R injury via phosphoinositide 3-kinase / protein kinase B / mammalian target of rapamycin (PI3K/AKT/mTOR) and c-Jun N-terminal kinase / mitogen-activated protein kinase (JNK/MAPK) pathways.
DISCUSSION: BBPP80-1a is a pectic polysaccharide. It exhibited the potential on cardioprotective effect through the activation of PI3K/AKT/mTOR survival pathway and the regulation of JNK/MAPK pathways.
METHODS: An acidic polysaccharide (BBPP80-1a) was isolated and purified from active BBPP80-1 fraction. Its structure was characterized based on some approaches. Its myocardial injury protection potential was performed in hypoxia/reoxygenation (H/R)-induced H9c2 cells injury model.
RESULTS: The molecular weight of BBPP80-1a was 164.91 kDa. The ratio of galacturonic acid (GalA) was 50.92% in methylation results. Further, nuclear magnetic resonance data suggested repetitive → 4)-α-D-GalpA-(1 → and → 4)-α-D-GalpA-6-OMe-(1 → residues occupied the homogalacturonan region of backbone. It had a highly porous, reticular, and heterogeneous microstructures. Moreover, BBPP80-1a can obviously protect H9c2 cells from H/R injury via phosphoinositide 3-kinase / protein kinase B / mammalian target of rapamycin (PI3K/AKT/mTOR) and c-Jun N-terminal kinase / mitogen-activated protein kinase (JNK/MAPK) pathways.
DISCUSSION: BBPP80-1a is a pectic polysaccharide. It exhibited the potential on cardioprotective effect through the activation of PI3K/AKT/mTOR survival pathway and the regulation of JNK/MAPK pathways.