Biogenic maturation of mineral-encrusted plastispheres overrides ecological drift to orchestrate multi-kingdom assembly and nitrogen reprogramming in agroecosystems.

The quantitative assembly rules and multi-kingdom functional gateways of the soil plastisphere remain poorly resolved. We integrated multi-omics with high-resolution surface deconvolution to decode the ecological signatures of weathered polyethylene (PE) films in intensive agroecosystems. Surface characterization revealed an interfacial metamorphosis where biogenic maturation facilitated the formation of a dense mineral organic crust, transforming inert polymers into reactive metabolic islands. Ecological modeling identifies a distinct departure from neutral assembly, demonstrating that niche-based selection driven by polymer surface remodeling overrides ecological drift in shaping microbial consortia. Machine learning and structural equation modeling identify ammonia oxidizing archaea as the primary functional keystone taxa orchestrating the global interactome. This archaeal-bacterial axis, regulated by protistan assemblages that exhibit significant topological densification, catalyzes a cross-kingdom metabolic relay synchronized with nitrogenous biomarker accumulation and intensified enzymatic mineralization. Functional decoupling across taxonomic domains reveals a metabolic lag where rapid deterministic recruitment outpaces steady-state nutrient stabilization. While promoting local alpha-diversity, the bacterial core exhibits structural fragmentation and elevated antagonistic interactions, signaling an asynchronous regime shift that may trigger positive priming effects on native soil organic carbon. These findings establish a mechanistic blueprint for how weathered PE residues redirect terrestrial nutrient fluxes via deterministic multi-kingdom assembly, pinpointing specific organo-mineral signatures and archaeal keystone taxa as critical targets for precision microbiome engineering in the Anthropocene.