Science (New York, N.Y.) · 2026
A DNA Typewriter records the cell lineage history of a mouse, from zygote to late organogenesis.
СтатьяНазвание и аннотация: Перевод пока не подготовлен
Обработка статьи · этапы, ошибки и управление
Загрузка состояния…
Ошибки и ограничения
История · было → стало
Состояние обновляется каждые 5 секунд, пока блок открыт. Процент показывается только там, где известен объём работы. Пауза останавливает продолжение этапа; текущий запрос к источнику может завершиться.
Yu Qi, Kim Haedong, Seidel Sophie, Acosta-Clark James F, Martin Beth K, O'Connor Kyle, Daza Riza M, Gasperini Molly, Nathans Jenny F, Lam Maggie, Gamo Elena, Vijay Kumar Shruthi, Kuo Lauren, Lalanne Jean-Benoît, Simeonov Kamen P, Pepper Marion, Trapnell Cole, Gray Jesse M, Choi Junhong, Qiu Chengxiang, Shendure Jay
Аннотация
Mammalian biology unfolds over time, within tissues and organs opaque to our eyes and instruments. We applied DNA Typewriter, a sequential molecular recorder, to record the cell lineage of a mouse over nearly two weeks of development. From one embryo, we reconstruct a time-calibrated, parsimony-supported, zygote-rooted phylogeny of 1.28 million transcriptionally profiled cells. A burst of editing unequivocally marks the daughters of the first cleavage, which serve as inline replicates. We quantify clonal dominance arising during gastrulation. Tree siblings share cell type far above chance; heterotypic siblings mark terminal differentiations. Temporal sweeps of clade co-occurrence recover a dated hierarchy of cell-type couplings; imputed labels for internal nodes recapitulate known state paths. A lineage-anchored ontogeny of mammalian development, long out of reach, is coming into view.