Higher Myocardial miR-1-3p, miR-30a-5p, miR-34a-5p, and Lower miR208a-3p Levels are Associated with Lower Age at the Time of Heart Transplantation.

Expression of microRNAs (miR-1-3p, miR-21-3p, miR-30a-5p, miR-34a-5p, miR-133a-3p, miR-208a-3p, and miR-423-5p) and mitochondrial unfolded protein response (UPRmt) genes (HSPA9, HSPD1, LONP1, OMA1, and YME1L) was investigated by qRT-PCR in six controls and 65 failing human hearts (33 ischemic cardiomyopathy (ICM), 28 dilated cardiomyopathy (DCM), 2 hypertrophic cardiomyopathy, and 1 non-compaction and restrictive cardiomyopathy each). Failing hearts had higher expression of miR-208a-3p, HSPA9, HSPD1, OMA1, YME1L and lower miR-1-3p expression compared to controls. Patients with higher miR-1-3p, miR-30a-5p, miR-34a-5p, (miR-423-5p exhibited a trend) and lower miR-208a-3p expression underwent heart transplantation or LVAD implantation earlier in life. DCM group had higher expression of miR-1-3p, miR-30a-5p, HSPD1, and LONP1 compared to the ICM group. Thus, myocardial expression of miR-1-3p, miR-30a-5p, miR-34a-5p, and miR-208a-3p could be associated with the HF progression; miR-208a-3p, HSPA9, HSPD1, OMA1, YME1L, and miR-1-3p could discriminate HF form control; and miR-1-3p, miR-30a-5p, HSPD1, and LONP1 could discriminate DCM from ICM.