Zhongguo zhen jiu = Chinese acupuncture & moxibustion · 2026
[Mechanism on protective effect of acupuncture combined with medication pretreatment for acute gastric ulcer rats based on metabolomics].
Аннотация на английскомСтатьяНазвание и аннотация: Перевод в очереди
Обработка статьи · этапы, ошибки и управление
Загрузка состояния…
Ошибки и ограничения
История · было → стало
Состояние обновляется каждые 5 секунд, пока блок открыт. Процент показывается только там, где известен объём работы. Пауза останавливает продолжение этапа; текущий запрос к источнику может завершиться.
Shi Jiaxin, Huo Jinnan, Bao Yongrui, Wang Shuai, Li Tianjiao, Zhang Minghui, Wang Yifan, Zheng Tiantian, Dong Baoqiang, Meng Xiansheng
Аннотация
OBJECTIVE: To observe the effects of acupuncture combined with medication pretreatment on gastric mucosal injury and serum metabolites in rats with acute gastric ulcer (GU) using ultra-high performance liquid chromatography-quadrupole-time of flight tandem mass spectrometry (UHPLC-Q-TOF-MS), and to explore the protective mechanism of this combined pretreatment for GU.
METHODS: Forty SPF-grade male SD rats were randomly divided into a blank group, a model group, an acupuncture combined with medication group (combined group) and an omeprazole group, with 10 rats in each group. In the omeprazole group, omeprazole solution was given by gavage administration at a dosage of 20 mg/kg; in the combined group, omeprazole solution was given by gavage administration, followed by acupuncture at bilateral "Zusanli"(ST36) 30 min later, the intervention was delivered once daily for 10 days in the two groups. After the intervention, except the blank group, acute GU model was established by intragastric administration of absolute ethanol in the other three groups. The gastric mucosal injury was observed, and the gastric mucosal injury index and injury inhibition rate were calculated. The morphology of gastric tissue was observed by HE staining, and the acute gastric mucosal injury score was assessed. The distribution of mucus on the gastric mucosal surface was observed by PAS staining. The levels of hexosamine (Hex) and phospholipid (PL) in the gastric tissue, as well as the levels of tumor necrosis factor-α (TNF-α) and interleukin-6 (IL-6) in the serum were detected by ELISA. The positive expression of B-cell lymphoma-2 (Bcl-2), Bcl-2 associated X protein (Bax),Occludin and zonula occludens-1 (ZO-1) in the gastric tissue was detected by immunohistochemistry. The endogenous metabolic profile of serum samples was analyzed by UHPLC-Q-TOF-MS to screen differential metabolites, and metabolic pathways were enriched using the MetaboAnalyst5.0 database. The protein expression of phosphatidylinositol 3-kinase(PI3K), protein kinase B (Akt), nuclear factor-κ B (NF-κ B), phosphorylated PI3K (p-PI3K), phosphorylated Akt (p-Akt) and phosphorylated NF-κ B (p-NF-κ B) in the gastric tissue was detected by Western blot.
RESULTS: Compared with the blank group, the gastric mucosal injury index and acute gastric mucosal injury score were increased in the model group (P<0.01).Compared with the model group, the gastric mucosal injury index and acute gastric mucosal injury scores were decreased(P<0.01), and the injury inhibition rate was increased in the combined group and the omeprazole group. In the model group,the mucus on the gastric mucosal surface was damaged and the mucosal structure was disrupted; in the combined group and the omeprazole group, the mucus was evenly distributed and the mucosal structure was relatively intact. Compared with the blank group, the levels of Hex and PL, the positive expression of Bcl-2 in gastric tissue, as well as the proportions of Occludin and ZO-1 positive cells in gastric mucosa were decreased (P<0.01); the serum levels of TNF-α and IL-6, the positive expression of Bax, and the protein expression of p-PI3K, p-Akt and p-NF-κ B in the gastric tissue were increased in the model group (P<0.01). Compared with the model group, the levels of Hex and PL in the gastric tissue, and the proportions of Occludin positive cells in the gastric mucosa were increased (P<0.01, P<0.05); the serum levels of TNF-α, the positive expression of Bax, and the protein expression of p-PI3K, p-Akt and p-NF-κ B in the gastric tissue were decreased (P<0.01,P<0.05) in the combined group and the omeprazole group. Compared with the model group, the positive expression of Bcl-2 in the gastric tissue, and the proportion of ZO-1 positive cells in the gastric mucosa were increased (P<0.01); the serum level of IL-6 was decreased (P<0.01) in the combined group. A total of 15 potential biomarkers were identified, mainly involved in metabolic pathways such as biosynthesis of phenylalanine, tyrosine and tryptophan, phenylalanine metabolism, alanine,aspartate and glutamate metabolism, and glutathione metabolism.
CONCLUSION: Acupuncture combined with medication pretreatment can enhance gastric barrier function, inhibit inflammatory response, inhibit apoptosis, and regulate metabolic disorders, thereby improving gastric mucosal injury in acute GU rats. Its mechanism may be associated with inhibiting the abnormal activation of the PI3K/Akt/NF-κ B pathway.
METHODS: Forty SPF-grade male SD rats were randomly divided into a blank group, a model group, an acupuncture combined with medication group (combined group) and an omeprazole group, with 10 rats in each group. In the omeprazole group, omeprazole solution was given by gavage administration at a dosage of 20 mg/kg; in the combined group, omeprazole solution was given by gavage administration, followed by acupuncture at bilateral "Zusanli"(ST36) 30 min later, the intervention was delivered once daily for 10 days in the two groups. After the intervention, except the blank group, acute GU model was established by intragastric administration of absolute ethanol in the other three groups. The gastric mucosal injury was observed, and the gastric mucosal injury index and injury inhibition rate were calculated. The morphology of gastric tissue was observed by HE staining, and the acute gastric mucosal injury score was assessed. The distribution of mucus on the gastric mucosal surface was observed by PAS staining. The levels of hexosamine (Hex) and phospholipid (PL) in the gastric tissue, as well as the levels of tumor necrosis factor-α (TNF-α) and interleukin-6 (IL-6) in the serum were detected by ELISA. The positive expression of B-cell lymphoma-2 (Bcl-2), Bcl-2 associated X protein (Bax),Occludin and zonula occludens-1 (ZO-1) in the gastric tissue was detected by immunohistochemistry. The endogenous metabolic profile of serum samples was analyzed by UHPLC-Q-TOF-MS to screen differential metabolites, and metabolic pathways were enriched using the MetaboAnalyst5.0 database. The protein expression of phosphatidylinositol 3-kinase(PI3K), protein kinase B (Akt), nuclear factor-κ B (NF-κ B), phosphorylated PI3K (p-PI3K), phosphorylated Akt (p-Akt) and phosphorylated NF-κ B (p-NF-κ B) in the gastric tissue was detected by Western blot.
RESULTS: Compared with the blank group, the gastric mucosal injury index and acute gastric mucosal injury score were increased in the model group (P<0.01).Compared with the model group, the gastric mucosal injury index and acute gastric mucosal injury scores were decreased(P<0.01), and the injury inhibition rate was increased in the combined group and the omeprazole group. In the model group,the mucus on the gastric mucosal surface was damaged and the mucosal structure was disrupted; in the combined group and the omeprazole group, the mucus was evenly distributed and the mucosal structure was relatively intact. Compared with the blank group, the levels of Hex and PL, the positive expression of Bcl-2 in gastric tissue, as well as the proportions of Occludin and ZO-1 positive cells in gastric mucosa were decreased (P<0.01); the serum levels of TNF-α and IL-6, the positive expression of Bax, and the protein expression of p-PI3K, p-Akt and p-NF-κ B in the gastric tissue were increased in the model group (P<0.01). Compared with the model group, the levels of Hex and PL in the gastric tissue, and the proportions of Occludin positive cells in the gastric mucosa were increased (P<0.01, P<0.05); the serum levels of TNF-α, the positive expression of Bax, and the protein expression of p-PI3K, p-Akt and p-NF-κ B in the gastric tissue were decreased (P<0.01,P<0.05) in the combined group and the omeprazole group. Compared with the model group, the positive expression of Bcl-2 in the gastric tissue, and the proportion of ZO-1 positive cells in the gastric mucosa were increased (P<0.01); the serum level of IL-6 was decreased (P<0.01) in the combined group. A total of 15 potential biomarkers were identified, mainly involved in metabolic pathways such as biosynthesis of phenylalanine, tyrosine and tryptophan, phenylalanine metabolism, alanine,aspartate and glutamate metabolism, and glutathione metabolism.
CONCLUSION: Acupuncture combined with medication pretreatment can enhance gastric barrier function, inhibit inflammatory response, inhibit apoptosis, and regulate metabolic disorders, thereby improving gastric mucosal injury in acute GU rats. Its mechanism may be associated with inhibiting the abnormal activation of the PI3K/Akt/NF-κ B pathway.