Journal of veterinary internal medicine · 2026
Validation of a point-of-care assay for simultaneous quantification of equine adrenocorticotropic hormone and corticotropin-like intermediate peptide.
СтатьяВалидационное исследованиеНазвание и аннотация: Перевод пока не подготовлен
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Hart Kelsey A, Berghaus Londa J, Harmon Ian
Аннотация
BACKGROUND: Plasma adrenocorticotropic hormone (ACTH) concentrations are used to diagnose equine pituitary pars intermedia dysfunction (PPID). The reference chemiluminescent immunoassay for immunoreactive ACTH (iACTH) cross-reacts with corticotropin-like intermediate peptide (CLIP), which is also abnormally high in PPID.
HYPOTHESIS/OBJECTIVES: To compare the performance of a novel point-of-care (POC) immunoassay that concurrently measures ACTH and CLIP against the reference iACTH assay.
ANIMALS: Twenty-eight university-owned horses (13 PPID, 15 non-PPID).
METHODS: Prospective observational study. Resting plasma hormone concentrations in all animals were measured by both methods in all seasons over 1 year, and after thyrotropin releasing hormone (TRH)-stimulation testing once. Bias of the POC-ACTH/CLIP assay was assessed using Spearman correlation analysis and Bland-Altman plots, and precision and linearity of the POC-ACTH/CLIP assay were determined.
RESULTS: For resting and TRH-stimulated ACTH concentrations, the POC-ACTH/CLIP assay and the reference method had good agreement overall (r ≥ 0.94; P < .001) and in PPID animals (r = 0.93, P < .001). Bias was greater in autumn (33.6-35.6 ± 89.5-96 pg/mL) compared to winter/spring (1.6-11.1 ± 8.4-29.6 pg/mL) and in samples with ACTH concentrations ≥ 90 pg/mL (87.8 ± 116.4 pg/mL). Intra- and inter-assay coefficients of variation for the POC-ACTH/CLIP assay were 8.47% and 14.1%, respectively, and assay linearity was excellent (R2 = 0.991).
CONCLUSIONS AND CLINICAL IMPORTANCE: These data demonstrate acceptable field performance for the POC-ACTH/CLIP assay and good agreement with the chemiluminescent iACTH immunoassay, with a larger bias in samples with higher hormone concentrations.
HYPOTHESIS/OBJECTIVES: To compare the performance of a novel point-of-care (POC) immunoassay that concurrently measures ACTH and CLIP against the reference iACTH assay.
ANIMALS: Twenty-eight university-owned horses (13 PPID, 15 non-PPID).
METHODS: Prospective observational study. Resting plasma hormone concentrations in all animals were measured by both methods in all seasons over 1 year, and after thyrotropin releasing hormone (TRH)-stimulation testing once. Bias of the POC-ACTH/CLIP assay was assessed using Spearman correlation analysis and Bland-Altman plots, and precision and linearity of the POC-ACTH/CLIP assay were determined.
RESULTS: For resting and TRH-stimulated ACTH concentrations, the POC-ACTH/CLIP assay and the reference method had good agreement overall (r ≥ 0.94; P < .001) and in PPID animals (r = 0.93, P < .001). Bias was greater in autumn (33.6-35.6 ± 89.5-96 pg/mL) compared to winter/spring (1.6-11.1 ± 8.4-29.6 pg/mL) and in samples with ACTH concentrations ≥ 90 pg/mL (87.8 ± 116.4 pg/mL). Intra- and inter-assay coefficients of variation for the POC-ACTH/CLIP assay were 8.47% and 14.1%, respectively, and assay linearity was excellent (R2 = 0.991).
CONCLUSIONS AND CLINICAL IMPORTANCE: These data demonstrate acceptable field performance for the POC-ACTH/CLIP assay and good agreement with the chemiluminescent iACTH immunoassay, with a larger bias in samples with higher hormone concentrations.