Extended-spectrum beta-lactamase-producing Escherichia coli and methicillin-resistant staphylococci originating from dog and cat urine in Japan, 2017-2024: annual proportions and prefecture-level distributions of submitted isolates.

We extracted extended-spectrum beta-lactamase (ESBL)-producing Escherichia coli and methicillin-resistant staphylococci (MRS) from dog and cat urine isolates submitted to a commercial veterinary laboratory in Japan from 2017 to 2024 and described their annual proportions and prefecture-level counts of submitted resistant isolates. The main trends were to significantly decrease the proportions of ESBL-production E. coli in both dogs and cats and MRS in dogs alone. The prefectures (Tokyo, Chiba, Saitama, Kanagawa, Kyoto, Aichi, Fukuoka, and Ibaraki) represented the larger numbers of resistant isolates submitted to the Sanritsu Zelkova Veterinary Laboratory database. Although this study was a single commercial laboratory-based surveillance, our observations may provide descriptive reference data for laboratory-based antimicrobial resistance surveillance and antimicrobial stewardship in companion animal practice.