Zhongguo zhen jiu = Chinese acupuncture & moxibustion · 2026
[Effect of electroacupuncture on microglial activation in the spinal dorsal horn of rats with postherpetic neuralgia based on the BDNF/TrkB pathway].
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Zhang Ronghua, Zou Jing
Аннотация
OBJECTIVE: To observe the effects of electroacupuncture (EA) on the brain-derived neurotrophic factor (BDNF)/tyrosine kinase receptor B (TrkB) pathway and microglial activation in the spinal dorsal horn of rats with postherpetic neuralgia (PHN).
METHODS: Five of 20 male SD rats were randomly assigned to the normal control (NC) group, and the remaining rats received intraperitoneal injection of resiniferatoxin solution (200 μg/kg) to establish a PHN model. Successfully modeled rats were randomly divided into a PHN group, an EA group, and an EA+BDNF group, with 5 rats in each group. The rats in the EA group received EA at ashi points, "Xinshu" (BL15), and "Danshu" (BL19) on the left side, with disperse-dense wave (2 Hz/15 Hz) and a current intensity of 1 mA, 30 min per session, once daily for 4 consecutive weeks. In the EA+BDNF group, 10 μg of recombinant rat BDNF protein solution was administered by intrathecal injection before each EA treatment. The mechanical withdrawal threshold was assessed using Von Frey analgesia meter, and the thermal withdrawal latency was evaluated using plantar thermal stimulation apparatus. Immunofluorescence staining was used to detect the positive expression of ionized calcium-binding adaptor molecule 1 (Iba-1), a marker of microglial activation, in the spinal dorsal horn. ELISA was used to measure interleukin-1β (IL-1β) and tumor necrosis factor-α (TNF-α) levels in the spinal dorsal horn. Western blot was used to detect the protein expression of BDNF and TrkB in the spinal dorsal horn.
RESULTS: Compared with the NC group, the PHN group showed decreased mechanical withdrawal threshold and increased thermal withdrawal latency (P<0.05), while positive Iba-1 expression, IL-1β and TNF-α levels, and BDNF and TrkB protein expression in the spinal dorsal horn were increased (P<0.05). Compared with the PHN group, the EA group showed increased mechanical withdrawal threshold and decreased thermal withdrawal latency (P<0.05), while positive Iba-1 expression, IL-1β and TNF-α levels, and BDNF and TrkB protein expression in the spinal dorsal horn were decreased (P<0.05). Compared with the EA+BDNF group, the EA group showed increased mechanical withdrawal threshold and decreased thermal withdrawal latency (P<0.05), while positive Iba-1 expression, IL-1β and TNF-α levels, and BDNF and TrkB protein expression in the spinal dorsal horn were all decreased (P<0.05).
CONCLUSION: EA could alleviate pain symptoms in rats with PHN, possibly by regulating the BDNF/TrkB pathway, inhibiting microglial activation in the spinal dorsal horn, and reducing inflammatory factor levels.
METHODS: Five of 20 male SD rats were randomly assigned to the normal control (NC) group, and the remaining rats received intraperitoneal injection of resiniferatoxin solution (200 μg/kg) to establish a PHN model. Successfully modeled rats were randomly divided into a PHN group, an EA group, and an EA+BDNF group, with 5 rats in each group. The rats in the EA group received EA at ashi points, "Xinshu" (BL15), and "Danshu" (BL19) on the left side, with disperse-dense wave (2 Hz/15 Hz) and a current intensity of 1 mA, 30 min per session, once daily for 4 consecutive weeks. In the EA+BDNF group, 10 μg of recombinant rat BDNF protein solution was administered by intrathecal injection before each EA treatment. The mechanical withdrawal threshold was assessed using Von Frey analgesia meter, and the thermal withdrawal latency was evaluated using plantar thermal stimulation apparatus. Immunofluorescence staining was used to detect the positive expression of ionized calcium-binding adaptor molecule 1 (Iba-1), a marker of microglial activation, in the spinal dorsal horn. ELISA was used to measure interleukin-1β (IL-1β) and tumor necrosis factor-α (TNF-α) levels in the spinal dorsal horn. Western blot was used to detect the protein expression of BDNF and TrkB in the spinal dorsal horn.
RESULTS: Compared with the NC group, the PHN group showed decreased mechanical withdrawal threshold and increased thermal withdrawal latency (P<0.05), while positive Iba-1 expression, IL-1β and TNF-α levels, and BDNF and TrkB protein expression in the spinal dorsal horn were increased (P<0.05). Compared with the PHN group, the EA group showed increased mechanical withdrawal threshold and decreased thermal withdrawal latency (P<0.05), while positive Iba-1 expression, IL-1β and TNF-α levels, and BDNF and TrkB protein expression in the spinal dorsal horn were decreased (P<0.05). Compared with the EA+BDNF group, the EA group showed increased mechanical withdrawal threshold and decreased thermal withdrawal latency (P<0.05), while positive Iba-1 expression, IL-1β and TNF-α levels, and BDNF and TrkB protein expression in the spinal dorsal horn were all decreased (P<0.05).
CONCLUSION: EA could alleviate pain symptoms in rats with PHN, possibly by regulating the BDNF/TrkB pathway, inhibiting microglial activation in the spinal dorsal horn, and reducing inflammatory factor levels.