Standardized Preparation and Characterization of Leukocyte-Rich and Leukocyte-Poor PRP Using PrecurCell® Kits.

OBJECTIVES

To compare the cellular composition of leukocyte-rich platelet-rich plasma (LR-PRP) and leukocyte-poor platelet-rich plasma (LP-PRP) prepared using PrecurCell® PRP and PrecurCell®-LP PRP kits, with particular emphasis on platelet enrichment, leukocyte content, and residual red blood cells.

MATERIAL AND

METHODS

Whole blood samples from healthy donors were processed using the two PRP preparation systems. Platelet concentration and red blood cell content were quantified using a hematology analyzer, and leukocyte content was assessed to characterize the resulting LR-PRP and LP-PRP preparations. Platelet enrichment was determined relative to baseline whole blood values.

RESULTS

The preparations achieved platelet concentrations up to six times the baseline whole blood values. Differences in leukocyte content distinguished the LR-PRP and LP-PRP preparations. No residual red blood cells were detected in the analyzed PRP samples.

CONCLUSION

The evaluated systems produced platelet-enriched preparations with distinct leukocyte profiles and no detectable residual red blood cells. These findings characterize the cellular differences between LR-PRP and LP-PRP and may inform further investigation of their application-specific biological effects. Functional and clinical studies are required to establish their therapeutic relevance.