Food research international (Ottawa, Ont.) · 2026

Collaborative studies on in vitro digestibility of dietary proteins based on the INFOGEST Quant protocol: toward international standardization.

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Egger Lotti, Blanco-Doval Ana, Brügger Cédric, Sousa Raquel, Abbühl Lychou, Hernández Marta Santos, Miralles Beatriz, Gómez-Marin Cristina, Macierzanka Adam, Szumała Patrycja, Kłosowska-Chomiczewska Ilona E, Mulet-Cabero Ana Isabel, Perez-Moral Natalia, Edwards Cathrina, Gwala Shannon, Fitzpatrick Conor, Rieder Anne, Lesmes Uri, Mashiah Leehen, Shani-Levi Carmit, Gonçalves Catarina, Amado Isabel Rodriguez, Nobre Clarisse, Pinheiro Ana C, Arranz Elena, Bot Francesca, O'Mahony James A, Grundy Myriam, Mayeur-Nickel Frédérique, Cruz Igor Pacheco, Sepúlveda-González Ailynne, Gonzales-Nieto Elva, Comi Irene, Abrankó László, Tormási Judit, González-González Felipe, Ruas-Madiedo Patricia, Ruiz Lorena, Faria Miguel Ângelo, Ramos Helena, Jiménez-Munoz Luis, Corredig Milena, Muleya Molly, Rosa-Sibakov Natalia, Broersen Kerensa, Goerdayal Soenita, Ménard Olivia, Cochet Marie-Françoise, Henry Gwénaële, Orlien Vibeke, Gouseti Ourania, Petersen Iben Lykke, Duque-Estrada Patricia, Olias Raquel, Delgado-Andrade Cristina, Simsek Sebnem, El Sedef Nehir, Karakaya Sibel, Rezzi Serge, Canarelli Stéphane, Cuénod Delphine, Mes Jurriaan, Boudewijn Anouk, Ogrodowczyk Anna, Suna Senem, Tedeschi Tullia, Cutroneo Sara, Accardo Francesca, Rosset Sophie, Dupont Didier, Brodkorb André, Recio Isidra, Portmann Reto

Аннотация

Based on recommendations by the Food and Agriculture Organization, an international standardization was launched for a static in vitro protein digestibility method, recently published as the INFOGEST Quant protocol. Based on the validated INFOGEST 2.0 method, the INFOGEST Quant protocol allows quantification of total protein digestibility, individual amino acid digestibility, and digestible indispensable amino acid score (DIAAS) calculation. Comparability of protein digestibility obtained with the INFOGEST Quant method was confirmed using identical substrates in vitro and in vivo. Method standardization within the International Dairy Federation and the International Organization for Standardization was initiated, and precision data were established in two international collaborative studies by over 25 laboratories. The three applied analytical endpoints were comparable. Robust precision estimates for o-phthalaldehyde and total nitrogen showed repeatability and reproducibility limits of 10% and 15%, respectively. Moreover, the protein digestibility of fresh dairy foods was higher than in freeze-dried samples, highlighting matrix and processing effects.

Предметные рубрики MeSH

Dietary Proteins Digestion Amino Acids Reproducibility of Results Animals International Cooperation Dairy Products o-Phthalaldehyde Humans Hydrolysis

Collaborative studies on in vitro digestibility of dietary proteins based on the INFOGEST Quant protocol: toward international standardization.

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Аннотация

Предметные рубрики MeSH