Clinical oral implants research · 2026
The Adjunctive Use of MSC Secretome for Guided Bone Regeneration: An Experimental In Vivo Investigation.
СтатьяНазвание и аннотация: Перевод пока не подготовлен
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Shanbhag Siddharth, Sanz-Esporrin Javier, Nuñez Javier, Luengo Fernando, Toledano-Osorio Manuel, Kampleitner Carina, Heimel Patrick, Schædl Barbara, Gonzalez F Javier Vela, Mohamed-Ahmed Samih, Gjerde Cecilie, Gruber Reinhard, Mustafa Kamal, Sanz Mariano
Аннотация
OBJECTIVE: To evaluate the efficacy of mesenchymal stromal cells' (MSC) secretome delivered as conditioned media (CM) as an adjunct to a xenograft (XG) and collagen membrane (MEM) for guided bone regeneration (GBR) in chronic non-contained mandibular defects in minipigs.
METHODS: Chronic bilateral mandibular defects were surgically created in seven minipigs. In a split-mouth design, defects were treated with either CM-functionalized (test) or native XG + MEM (control) and evaluated after 4 and 12 weeks. New bone formation was assessed using in vivo computed tomography (CT) and ex vivo micro-CT, histology and immunohistochemistry (IHC). Quantitative CT, micro-CT and histomorphometric data were statistically analysed.
RESULTS: No significant differences in new bone formation were observed between the test and control group at either timepoint. These findings could be partly explained by a high intrinsic healing capacity and continual growth of the animals. Minimal residual XG material was detected in both groups at 4 and 12 weeks. When present, these remnants were associated with multinucleated Cathepsin K-positive cells (IHC) with no observable differences between the groups.
CONCLUSION: The adjunctive use of MSC secretome in combination with XG and MEM did not improve GBR outcomes in chronic non-contained mandibular defects in the present minipig model. The suitability of this animal model for experimental GBR may be questionable.
METHODS: Chronic bilateral mandibular defects were surgically created in seven minipigs. In a split-mouth design, defects were treated with either CM-functionalized (test) or native XG + MEM (control) and evaluated after 4 and 12 weeks. New bone formation was assessed using in vivo computed tomography (CT) and ex vivo micro-CT, histology and immunohistochemistry (IHC). Quantitative CT, micro-CT and histomorphometric data were statistically analysed.
RESULTS: No significant differences in new bone formation were observed between the test and control group at either timepoint. These findings could be partly explained by a high intrinsic healing capacity and continual growth of the animals. Minimal residual XG material was detected in both groups at 4 and 12 weeks. When present, these remnants were associated with multinucleated Cathepsin K-positive cells (IHC) with no observable differences between the groups.
CONCLUSION: The adjunctive use of MSC secretome in combination with XG and MEM did not improve GBR outcomes in chronic non-contained mandibular defects in the present minipig model. The suitability of this animal model for experimental GBR may be questionable.