DNA-polyphenol wet-adhesive hydrogel for rapid hemostasis.

Rapid hemostasis under wet and dynamic conditions remains a major challenge for wound management. Herein, a DNA-polyphenol wet-adhesive hydrogel was developed by integrating DNA with tannic acid (TA), dopamine (DOPA), and mesoporous bioactive glass (MBG). The resulting hydrogel exhibited robust wet adhesion, enabling effective sealing on various substrates and biological tissues. The integration of DNA, polyphenol components, and MBG endowed the hydrogel with enhanced adhesive properties, with an adhesive strength exceeding 35 kPa after prolonged immersion. In vitro studies demonstrated enhanced coagulation-related performance, including a reduced blood clotting index and shortened clotting time, together with high fluid absorption capacity. In vivo studies in rat liver injury, rat tail amputation, and rabbit femoral artery models further demonstrated effective hemostatic performance, with reduced blood loss and shortened hemostasis time, even under high-pressure bleeding conditions. In addition, the hydrogel exhibited good cytocompatibility, distinct in vitro release behavior, and progressive in vivo resorption with favorable tissue compatibility. These results suggest that the DNA-polyphenol hydrogel is a promising biomacromolecule-based material for rapid hemostasis and wound sealing applications.