Proteomic profile of human dental follicle stem cells and apical papilla stem cells.

Dental stem cells have great potential in clinical practice as an adult mesenchymal stem cell, such as dental follicle and the apical papilla, have strong proliferation and differentiation characteristics. The developmental relevance and discrimination of them in the niche is not clear, which limits their application scenarios. The aim of this study was to investigate the intrinsical differences in cellular contents of DFSCs and SCAP by Tandem mass tag (TMT) labeling quantitative proteomics. Cell lysates were labeled and tracked by the combined use of TMT and LC-MS/MS. A total of 1622 proteins were detected, of which 421 were different and 12 were significantly up-regulated and 4 were significantly down-regulated. The results of proteomics support the application of stem cells in the treatment of neurodegenerative diseases such as Huntington's disease, Alzheimer's disease, Parkinson's disease and so on. The difference is related to cell proliferation and protection of neurons from inflammation and autophagy damage. Highly expressed proteins predict the special ability of DFSCs to stably proliferate and differentiate through CD13, MARCKS, and PAST1. The strong immune stability of SCAP is supported by NPC1.This study expands our understanding on the molecular mechanisms of tooth development and regeneration, and provide basic support for dental stem cells in clinical applications such as neurological and immune diseases.