Fabrication of electrospun nanofibrous PVA/PEO/alginate sulfate water-stable mats via a gentle, non-toxic freeze-thawing cross-linking method as a potential drug delivery system.

This communication introduces a protocol for the fabrication of PVA/PEO/alginate sulfate electrospun mats cross-linked using a green, non-toxic, and gentle freeze-thaw approach. We present an electrospinning solution composition that yields homogeneous, bead-free fibres that cross-link tightly during repeated cycles of freezing and thawing, producing a hydrogel-like mat that is stable for at least 4 weeks under aqueous conditions at 37 °C.