Acta parasitologica · 2026
Seasonal Distribution and Genetic Diversity of Enterocytozoon bieneusi and Giardia duodenalis in Dairy Cattle from an Intensive Farm in Inner Mongolia, China.
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Liu Yi-Yang, Zhao Chun-Xue, Jia Jun-Tao, Liu Zhen, Liu Tao, Zhang Chun-Hao, Lv Yue-Rong, Xue Shi-Yuan, Ding Yu-Lin, Zhao Li, Liu Yong-Hong
Аннотация
PURPOSE: Enterocytozoon bieneusi is a microsporidium, and Giardia duodenalis is an important intestinal pathogen, both infect a wide range of hosts, including cattle. This study investigated the seasonal distribution and genetic diversity of these pathogens in dairy cattle in Hohhot, Inner Mongolia, China.
METHODS: A total of 505 fecal samples were collected from an intensive dairy farm during different seasonal sampling periods in 2024. Genomic DNA was extracted, and the internal transcribed spacer region of E. bieneusi and the small subunit (SSU) rRNA, beta-giardin (bg), glutamate dehydrogenase (gdh), and triosephosphate isomerase (tpi) genes of G. duodenalis were amplified by nested polymerase chain reaction and sequenced.
RESULTS: Overall prevalence of E. bieneusi and G. duodenalis was 26.53% and 29.90%, respectively. E. bieneusi predominated in spring and winter, whereas G. duodenalis predominated in autumn and winter. The genotypes of E. bieneusi were BEB4, J, and Horse1 alongside the HHOT1-12, with genotype BEB4 being dominant. G. duodenalis assemblages were identified as A, B, and E, with assemblage E being dominant.
CONCLUSIONS: To the best of our knowledge, this study reports the first systematic investigation of the seasonal distribution of E. bieneusi and G. duodenalis in cattle in Inner Mongolia, providing important baseline data for understanding their epidemiology.
METHODS: A total of 505 fecal samples were collected from an intensive dairy farm during different seasonal sampling periods in 2024. Genomic DNA was extracted, and the internal transcribed spacer region of E. bieneusi and the small subunit (SSU) rRNA, beta-giardin (bg), glutamate dehydrogenase (gdh), and triosephosphate isomerase (tpi) genes of G. duodenalis were amplified by nested polymerase chain reaction and sequenced.
RESULTS: Overall prevalence of E. bieneusi and G. duodenalis was 26.53% and 29.90%, respectively. E. bieneusi predominated in spring and winter, whereas G. duodenalis predominated in autumn and winter. The genotypes of E. bieneusi were BEB4, J, and Horse1 alongside the HHOT1-12, with genotype BEB4 being dominant. G. duodenalis assemblages were identified as A, B, and E, with assemblage E being dominant.
CONCLUSIONS: To the best of our knowledge, this study reports the first systematic investigation of the seasonal distribution of E. bieneusi and G. duodenalis in cattle in Inner Mongolia, providing important baseline data for understanding their epidemiology.