Complete genome sequence of Enterococcus faecium strain GB_C_05 with potential characteristics applicable as a bacteriocin-producing probiotic feed additive.

The whole genome of Enterococcus faecium GB_C_05, a strain isolated from Sikhye, a traditional Korean rice beverage, was successfully sequenced and analyzed using Oxford Nanopore Technologies. The complete genome sequence of GB_C_05 contains a circular chromosome with a total length of 2,575,440 base pairs (bp) and a guanine + cytosine (GC) content of 38.2%, along with one circular plasmid, which has a length of 230,283 bp and a GC content of 35.2%. Annotation of the GB_C_05 genome revealed 2,756 protein-coding sequences (CDSs), 70 tRNAs, and 18 rRNAs on the chromosome. Notably, CDSs related to bacteriocin synthesis (Enterocin) and carbohydrate metabolism (α-galactosidase, β-glucosidase, and α-L-arabinofuranosidase), as well as genes potentially involved in probiotic-associated functions such as adhesion and colonization, were identified. This comprehensive study presents the complete genome sequence of E. faecium GB_C_05, providing insight into the diverse additives utilized in animal farming to enhance nutritional quality and livestock productivity.