Metabolomic and lipidomic signatures of Helicobacter pylori infection: Unveiling biochemical pathways associated with neurodegeneration.

Our study employed an integrated lipidomics and metabolomics approach to elucidate Helicobacter pylori-driven metabolic perturbations along the gut-brain axis. H. pylori infection was established in gastric epithelial (AGS) cells, and the resulting conditioned media (secretome) was collected and exposed to neuronal (IMR-32) cells. Gastric, neuronal, and secretome samples were then harvested for the extraction of metabolites and lipids. Comprehensive molecular profiling was performed using high-resolution LC-MS/HRMS. The acquired data were analysed through lipidomics and metabolomics pipelines to identify significantly altered metabolites and lipid species. Subsequent statistical and pathway enrichment analyses uncovered dysregulated metabolic pathways. Finally, network-based interaction analysis integrated disease-associated metabolites to identify key molecular hubs potentially linking H. pylori infection to neuronal metabolic dysfunction and neurodegenerative processes.