Genome sequence and phylogenetic characterization of a Fusarium oxysporum mitovirus 2 variant from the Canary Islands, Spain.

We report the complete coding genome sequence of Fusarium oxysporum mitovirus 2-like (FoMV2-like; GenBank accession PZ600027; BioProject ID 3036262), a variant of FoMV2, identified in a Fusarium oxysporum strain isolated from Phytolacca dioica (ombú) in the Canary Islands, Spain. The positive-sense single-stranded RNA genome is 2,363 nucleotides in length and contains a single open reading frame encoding a 713-amino acid RNA-dependent RNA polymerase (RdRp). Pairwise genome comparisons revealed 87.33% nucleotide identity with FoMV2, while the predicted RdRp shared 82.19% amino acid identity with its closest relative. Phylogenetic analysis based on RdRp amino acid sequences placed FoMV2-like within a strongly supported clade with FoMV2 (ultrafast bootstrap = 100), supporting its classification as a variant rather than a distinct species according to current ICTV demarcation criteria for members of the family Mitoviridae. This study documents the first occurrence of a FoMV2-related sequence in a F. oxysporum isolate associated with P. dioica and represents the first record of this viral lineage in the Canary Islands, Spain. The sequence reported here contributes to the growing genomic diversity of mitoviruses associated with Fusarium species and provides a resource for future comparative and evolutionary studies.