MedElevator
Войти в исследования Investigative ophthalmology & visual science · 2026
The A53T Parkinson's Disease Mouse Model Shows Abnormal Outer Retinal OCT Metabolic Biomarker Patterns.
СтатьяНазвание и аннотация: Перевод пока не подготовлен
Обработка статьи · этапы, ошибки и управление
Загрузка состояния…
Ошибки и ограничения
История · было → стало
Состояние обновляется каждые 5 секунд, пока блок открыт. Процент показывается только там, где известен объём работы. Пауза останавливает продолжение этапа; текущий запрос к источнику может завершиться.
Ataman Ehimenmen S, Nguyen Christine T O, Finkelstein David I, Wong Vickie H Y, Hoang Anh, Lee Pei Ying, Soetedjo James, Klopp Brianna, Landowski Michael, Podolsky Robert H, Roberts Robin, Berkowitz Bruce A
Аннотация
PURPOSE: The purpose of this study was to test for abnormal optical coherence tomography (OCT) metabolic biomarker patterns in the A53T Parkinson's disease model, which accumulates α-synuclein in photoreceptor inner segments before overt motor symptoms.
METHODS: Four-to-6-month-old transgenic A53T homozygous mice and B6C3H wild-type (WT) littermate controls were evaluated using 2 OCT metabolic biomarkers. The first was the external limiting membrane (ELM) to retinal pigment epithelium (RPE) thickness, a proxy for subretinal space acidification and retinoid deficiency. The second biomarker was the sub-ELM hyper-reflective band profile shape minor:major aspect ratio, a proxy for mitochondrial configuration within photoreceptor inner segments. We also tested for outer nuclear layer (ONL) thinning, OCT metabolic biomarker sensitivity to light-dark conditions, and nicotinamide nucleotide transhydrogenase (Nnt) expression.
RESULTS: A53T mice showed inferior and superior subnormal ELM-RPE thickness, and a supranormal sub-ELM hyper-reflective band profile shape aspect ratio in the inferior but not the superior retina. Central retinal ONL thickness was unremarkable between groups. Compared to light, dark-adaptation evoked contraction in ELM-RPE thickness and an increase in sub-ELM hyper-reflective band profile shape aspect ratio in both cohorts. In addition, B6C3H control mice expressed functional Nnt.
CONCLUSIONS: The abnormal OCT metabolic biomarker patterns raise the possibility of central outer retinal dysmetabolism in A53T mice, despite normal ONL thickness. Light-dark results show that ELM-RPE contraction can be caused by increased metabolic demand in the A53T mouse model.
METHODS: Four-to-6-month-old transgenic A53T homozygous mice and B6C3H wild-type (WT) littermate controls were evaluated using 2 OCT metabolic biomarkers. The first was the external limiting membrane (ELM) to retinal pigment epithelium (RPE) thickness, a proxy for subretinal space acidification and retinoid deficiency. The second biomarker was the sub-ELM hyper-reflective band profile shape minor:major aspect ratio, a proxy for mitochondrial configuration within photoreceptor inner segments. We also tested for outer nuclear layer (ONL) thinning, OCT metabolic biomarker sensitivity to light-dark conditions, and nicotinamide nucleotide transhydrogenase (Nnt) expression.
RESULTS: A53T mice showed inferior and superior subnormal ELM-RPE thickness, and a supranormal sub-ELM hyper-reflective band profile shape aspect ratio in the inferior but not the superior retina. Central retinal ONL thickness was unremarkable between groups. Compared to light, dark-adaptation evoked contraction in ELM-RPE thickness and an increase in sub-ELM hyper-reflective band profile shape aspect ratio in both cohorts. In addition, B6C3H control mice expressed functional Nnt.
CONCLUSIONS: The abnormal OCT metabolic biomarker patterns raise the possibility of central outer retinal dysmetabolism in A53T mice, despite normal ONL thickness. Light-dark results show that ELM-RPE contraction can be caused by increased metabolic demand in the A53T mouse model.