Pregnancy outcomes after amniocentesis in ART-Conceived versus spontaneously conceived twin pregnancies: A single-center retrospective study.

ObjectiveTo compare the pregnancy outcomes following amniocentesis in twin pregnancies conceived with assisted reproduction technology (ART) versus spontaneously conceived twins.MethodsIn this retrospective study, the 40case records of twin pregnancies in which amniocentesis was performed at HuizhouCentral People's Hospital between 2021 and 2024 were reviewed. Pregnancy outcomes and neonatal outcomes of twin pregnancies were compared between the ART group (n = 25)and spontaneous twin pregnancy group(n = 15).ResultsThere was no statistically significant difference in pregnancy loss rate between the groups(4.00% vs. 6.67%, P = 1.000). The pregnancy loss rate before 24 weeks of gestation in the two groups were 4.00% and 6.67%, respectively (P = 1.000). No pregnancy loss occurred within 4 weeks after the amniocentesis in bothgroup. The preterm birthrate before 37weeks of gestation in the two groups were 76.00% and 46.67%, respectively, and showed no significant difference (P =0.089).There were significant differences between-group in birth weight and Apgar score at 10 min (both P< 0.05), while there were no significant differences in low birth weight (<2500 g), Apgar score at 1 min, Apgar score at 5 min, and neonatal/pediatric department transfer (all P> 0.05).ConclusionNo statistically significant increase in the risk of pregnancy loss after amniocentesis was observed among ART-conceived twin pregnancies compared with spontaneously conceived twin pregnancies.However, they should not be interpreted as evidence of equivalence or absence of a clinically meaningful difference.Given the limited sample size, these findings should be confirmed in larger, multicenter studies.