Bottlenecks in Proteomics: An Addendum.

Mass spectrometry (MS) is the core for advanced methods in proteomic experiments. When effectively used, proteomics may provide extensive information about proteins and their post-translational modifications, as well as their interaction partners. However, there are also many problems that one can encounter during a proteomic experiment, including, but not limited to, sample preparation, sample fractionation, sample analysis, data analysis and interpretation, and biological significance. Here, we discuss some of the problems that researchers should be aware of when performing a proteomic experiment.