Parkinsonism & related disorders · 2026
Up-regulation of tRNA-derived miR-1274b in tears of Parkinson's disease patients.
СтатьяНазвание и аннотация: Перевод в очереди
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Salvi Erika, Vannetiello Ilaria, Cazzaniga Federico Angelo, Ciullini Arianna, Demleitner Antonia F, Andreasi Nico Golfré, Tomé Simone, Elia Antonio Emanuele, Leta Valentina, Lingor Paul, Eleopra Roberto, Catania Marcella, Devigili Grazia, Moda Fabio
Аннотация
BACKGROUND: Parkinson's disease (PD) is a complex neurodegenerative disorder characterized by motor and non-motor symptoms. Early diagnosis remains challenging due to the lack of reliable, non-invasive biomarkers. Here, we explored the potential of tear fluid (TF) as a diagnostic source by profiling its microRNA (miRNA) content.
METHODS: The study included a discovery (8 PD, 7 healthy controls (HC)) and a replication cohort (9 PD, 9 HC). miRNA expression was assessed using TaqMan Human MicroRNA arrays. Independent in-silico validation was conducted using a qPCR-based miRNA array dataset comprising pooled cDNA samples from controls (n = 10) and PD patients (n = 29). The relationship between microRNA expression and composite clinical scores in PD patients was explored. Target gene prediction, pathway enrichment, and interaction network analyses were performed.
RESULTS: The t-RNA-derived miR-1274b was consistently upregulated in the TF of PD patients across the discovery and replication cohorts, with additional in-silico support from the independent dataset. Increased expression was particularly evident in a PD subgroup characterized by combined non-motor autonomic symptoms. Predicted target genes were associated with neurodegeneration and cellular dysfunction. Over-representation analysis highlighted pathways related to negative regulation of synaptic transmission, while network analysis revealed interactions between miRNA targets and key PD-related genes, supporting a potential role in disease mechanisms.
CONCLUSIONS: Our findings identify miR-1274b as a TF-based miRNA associated with PD, supporting its potential as a non-invasive biomarker. Further studies with larger cohorts and other neurodegenerative diseases are needed to validate these results and explore their clinical applicability.
METHODS: The study included a discovery (8 PD, 7 healthy controls (HC)) and a replication cohort (9 PD, 9 HC). miRNA expression was assessed using TaqMan Human MicroRNA arrays. Independent in-silico validation was conducted using a qPCR-based miRNA array dataset comprising pooled cDNA samples from controls (n = 10) and PD patients (n = 29). The relationship between microRNA expression and composite clinical scores in PD patients was explored. Target gene prediction, pathway enrichment, and interaction network analyses were performed.
RESULTS: The t-RNA-derived miR-1274b was consistently upregulated in the TF of PD patients across the discovery and replication cohorts, with additional in-silico support from the independent dataset. Increased expression was particularly evident in a PD subgroup characterized by combined non-motor autonomic symptoms. Predicted target genes were associated with neurodegeneration and cellular dysfunction. Over-representation analysis highlighted pathways related to negative regulation of synaptic transmission, while network analysis revealed interactions between miRNA targets and key PD-related genes, supporting a potential role in disease mechanisms.
CONCLUSIONS: Our findings identify miR-1274b as a TF-based miRNA associated with PD, supporting its potential as a non-invasive biomarker. Further studies with larger cohorts and other neurodegenerative diseases are needed to validate these results and explore their clinical applicability.