Frontiers in immunology · 2026
Oligopeptide-binding protein A is a potential target for combating Salmonella infection.
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Yoshii Ken, Suzuki Koichiro, Adachi Jun, Piao Zhenyu, Node Eri, Liu Zilai, Nagatake Takahiro, Hosomi Koji, Kunisawa Jun
Аннотация
INTRODUCTION: Salmonella infection remains a pervasive diarrheal illness, particularly affecting populations in developing countries. The lack of an effective vaccine against non-typhoidal Salmonella is attributable to antigenic diversity among serotypes, incomplete understanding of protective immune mechanisms, and the difficulty in identifying conserved antigens capable of eliciting broad cross-protective immunity.
METHODS: To identify conserved Salmonella antigens, we established a Salmonella-specific monoclonal antibody library using heat-killed bacteria. Candidate antigens recognized by monoclonal antibodies were characterized, and their functional relevance was evaluated through analyses of bacterial growth, respiratory activity, and protective effects in a murine Salmonella infection model.
RESULTS: We identified L2-1H11, a monoclonal antibody that recognizes OppA, a conserved antigen present across multiple Salmonella serotypes. L2-1H11 was associated with suppression of bacterial growth and respiratory activity and attenuated enteritis pathology in Salmonella-infected mice.
CONCLUSION: These findings identify OppA as a conserved Salmonella antigen and suggest that it may represent a promising target for the development of subcutaneous and oral vaccines against Salmonella.
METHODS: To identify conserved Salmonella antigens, we established a Salmonella-specific monoclonal antibody library using heat-killed bacteria. Candidate antigens recognized by monoclonal antibodies were characterized, and their functional relevance was evaluated through analyses of bacterial growth, respiratory activity, and protective effects in a murine Salmonella infection model.
RESULTS: We identified L2-1H11, a monoclonal antibody that recognizes OppA, a conserved antigen present across multiple Salmonella serotypes. L2-1H11 was associated with suppression of bacterial growth and respiratory activity and attenuated enteritis pathology in Salmonella-infected mice.
CONCLUSION: These findings identify OppA as a conserved Salmonella antigen and suggest that it may represent a promising target for the development of subcutaneous and oral vaccines against Salmonella.