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Войти в исследования Environmental toxicology and pharmacology · 2026
Urinary KIM-1 Transcript Detectability in Workers Chronically Exposed to Arsenic and Antimony in an Industrial Area of Central Chile: A Cross-Sectional Pilot Study.
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Roldán Nicole, Tognarelli Javier, Castro Martín, Miranda Rodrigo, Gutiérrez Javiera, Riveros Valeria, Ruiz Catalina, Verdugo Marcelo, Gonzalez Alexis A, Quiroz Waldo, Cantillana Carolina, Gigoux Juan Pablo, Muñoz Leonel
Аннотация
BACKGROUND: Chronic arsenic (As) and antimony (Sb) exposure causes renal toxicity in experimental models, but early molecular indicators of tubular stress in humans remain uncharacterised.
METHODS: We assessed 12 male workers from Puchuncaví, Chile. Urinary and blood As and Sb were quantified by hydride-generation atomic fluorescence spectrometry (HG-AFS). Urinary KIM-1, NGAL, CTGF and PAI-1 transcripts were assessed by RT-qPCR, with 18S rRNA confirming amplifiability; detection required Ct < 35 with a single melting peak.
RESULTS: Ten samples passed quality control. KIM-1 was detected in 5 of 10 participants (50%); NGAL, CTGF and PAI-1 were detected sporadically. Serum creatinine and blood urea nitrogen remained within reference ranges in all but one participant. Detection was not associated with urinary or blood metalloid concentrations (Mann-Whitney U, p ≥ 0.11).
CONCLUSION: Urinary KIM-1 transcripts are detectable in workers chronically exposed to As and Sb despite normal conventional renal function. Establishing biomarker performance requires larger studies with unexposed reference populations.
METHODS: We assessed 12 male workers from Puchuncaví, Chile. Urinary and blood As and Sb were quantified by hydride-generation atomic fluorescence spectrometry (HG-AFS). Urinary KIM-1, NGAL, CTGF and PAI-1 transcripts were assessed by RT-qPCR, with 18S rRNA confirming amplifiability; detection required Ct < 35 with a single melting peak.
RESULTS: Ten samples passed quality control. KIM-1 was detected in 5 of 10 participants (50%); NGAL, CTGF and PAI-1 were detected sporadically. Serum creatinine and blood urea nitrogen remained within reference ranges in all but one participant. Detection was not associated with urinary or blood metalloid concentrations (Mann-Whitney U, p ≥ 0.11).
CONCLUSION: Urinary KIM-1 transcripts are detectable in workers chronically exposed to As and Sb despite normal conventional renal function. Establishing biomarker performance requires larger studies with unexposed reference populations.