Cryopreservation Method for Aurantiochytrium limacinum.

Aurantiochytrium limacinum is a marine protist that produces high levels of omega-3 fatty acid DHA and is thus of biotechnological importance. Here, we describe a cryopreservation technique to preserve A. limacinum at two different temperatures (-80°C) and liquid nitrogen (-196°C). At -80°C, cells were preserved in 25% (v/v) glycerol; at -196°C (liquid nitrogen), in 10% (v/v) methanol, 15% (v/v) glycerol, or 15% (v/v) dimethyl sulfoxide. The cells were stable for up to 12 months of storage. The method preserves the genetic integrity and functionality of the cells for biotechnological applications.