International urogynecology journal · 2025
Mast Cells and Interstitial Cystitis/Bladder Pain Syndrome Revisited.
СтатьяПоддержка службы здравоохранения СШАПоддержка NIH: внешняя программаНазвание и аннотация: Перевод в очереди
Обработка статьи · этапы, ошибки и управление
Загрузка состояния…
Ошибки и ограничения
История · было → стало
Состояние обновляется каждые 5 секунд, пока блок открыт. Процент показывается только там, где известен объём работы. Пауза останавливает продолжение этапа; текущий запрос к источнику может завершиться.
Ruetten Hannah, Ritts Rory, Namugosa Mary, Li Wencheng, Evans Robert, Badlani Gopal, Walker Stephen J
Аннотация
INTRODUCTION AND HYPOTHESIS: There is significant variation in interstitial cystitis/bladder pain syndrome (IC/BPS) biopsy processing and reporting. The objective of this study was to review pathology reports from a large IC/BPS patient cohort to identify differences in findings. We hypothesize that variation in IC/BPS bladder biopsy reporting might be most frequent when it comes to mast-cell counts.
METHODS: We performed a retrospective analysis of 461 diagnostic pathology reports collected from our IRB-approved prospective study of patients diagnosed with IC/BPS at the Urology Clinic at Wake Forest Baptist Hospital from October 2011 to July 2023 (IRB00018552). Data were assigned as continuous or categorical variables. Groups were compared using Student's t test, Mann-Whitney, or Chi-squared tests.
RESULTS: Staining strategy for mast-cell visualization differed between pathologists and included in order of frequency mast-cell tryptase (TPSAB1), CD117 (KIT), unspecified stain, a combination of stains, and toluidine blue. Mast-cell count was reported as a single number, range, or qualitatively. Pathologists used units of high-powered field (HPF), mm2, or did not specify. As expected, average mast-cell count per HPF was significantly lower than per mm2 across all stains (p < 0.0001). Average count with KIT was significantly lower than TPSAB1 (p < 0.0001). This trend remained significant when considering only KIT and TPSAB1 counts per HPF (p = 0.0007). Additionally, reports identified squamous metaplasia, acute inflammation, and/or chronic inflammation.
CONCLUSIONS: There is a lack of standardization regarding histological analysis of bladder biopsies from patients with IC/BPS, leading to inconsistent data and confusion surrounding the significance of pathology report findings.
METHODS: We performed a retrospective analysis of 461 diagnostic pathology reports collected from our IRB-approved prospective study of patients diagnosed with IC/BPS at the Urology Clinic at Wake Forest Baptist Hospital from October 2011 to July 2023 (IRB00018552). Data were assigned as continuous or categorical variables. Groups were compared using Student's t test, Mann-Whitney, or Chi-squared tests.
RESULTS: Staining strategy for mast-cell visualization differed between pathologists and included in order of frequency mast-cell tryptase (TPSAB1), CD117 (KIT), unspecified stain, a combination of stains, and toluidine blue. Mast-cell count was reported as a single number, range, or qualitatively. Pathologists used units of high-powered field (HPF), mm2, or did not specify. As expected, average mast-cell count per HPF was significantly lower than per mm2 across all stains (p < 0.0001). Average count with KIT was significantly lower than TPSAB1 (p < 0.0001). This trend remained significant when considering only KIT and TPSAB1 counts per HPF (p = 0.0007). Additionally, reports identified squamous metaplasia, acute inflammation, and/or chronic inflammation.
CONCLUSIONS: There is a lack of standardization regarding histological analysis of bladder biopsies from patients with IC/BPS, leading to inconsistent data and confusion surrounding the significance of pathology report findings.