Journal of pediatric surgery · 2026
Effects of growth factor application on germ cell architecture and oxidative stress handling in a rat model of testicular torsion-detorsion.
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Menon Revathy, Saxena Rahul, Elhence Poonam, Nayak Prasunpriya, Sharma Tripti, Pathak Manish, Rathod Kirtikumar J, Jadhav Avinash S, Nayak Shubhalakshmi, Sinha Arvind
Аннотация
PURPOSE: Testicular torsion is a surgical emergency in paediatric population, in which delayed intervention often leads to irreversible ischaemic injury. Oxidative stress plays a key role in testicular damage. Although several experimental therapies have been explored, the effect of local growth hormone (GH) application needs to be further defined. This study evaluated the impact of local GH on testicular morphology and oxidative stress parameters following torsion-detorsion in a rat model.
METHODS: Eighteen male Wistar rats were randomly assigned to three groups (n = 6 each): sham, torsion, and local GH. Torsion was induced in the torsion and GH groups, followed by detorsion. In the GH group, 2 IU of recombinant human GH loaded onto a gelatin hydrogel was applied locally to the testis immediately after detorsion. After 24 h, the testes were harvested for histopathological and biochemical analyses. Histological injury was graded using the Cosentino score, and antioxidant parameters, including superoxide dismutase, catalase, glutathione peroxidase, glutathione reductase, and reduced glutathione, were measured spectrophotometrically.
RESULTS: The median Cosentino score was significantly higher in the torsion group than in the sham and GH-treated groups, indicating improved preservation of testicular architecture following GH application. In contrast, the oxidative stress-handling capacity was impaired in the GH group, with significantly reduced levels of reduced glutathione, catalase, and glutathione peroxidase, along with diminished total superoxide handling capacity.
CONCLUSION: Local application of growth hormone after torsion-detorsion preserved testicular architecture and reduced histological injury compared with the torsion group. This protective effect was not associated with an increase in the antioxidant enzyme activity.
METHODS: Eighteen male Wistar rats were randomly assigned to three groups (n = 6 each): sham, torsion, and local GH. Torsion was induced in the torsion and GH groups, followed by detorsion. In the GH group, 2 IU of recombinant human GH loaded onto a gelatin hydrogel was applied locally to the testis immediately after detorsion. After 24 h, the testes were harvested for histopathological and biochemical analyses. Histological injury was graded using the Cosentino score, and antioxidant parameters, including superoxide dismutase, catalase, glutathione peroxidase, glutathione reductase, and reduced glutathione, were measured spectrophotometrically.
RESULTS: The median Cosentino score was significantly higher in the torsion group than in the sham and GH-treated groups, indicating improved preservation of testicular architecture following GH application. In contrast, the oxidative stress-handling capacity was impaired in the GH group, with significantly reduced levels of reduced glutathione, catalase, and glutathione peroxidase, along with diminished total superoxide handling capacity.
CONCLUSION: Local application of growth hormone after torsion-detorsion preserved testicular architecture and reduced histological injury compared with the torsion group. This protective effect was not associated with an increase in the antioxidant enzyme activity.