Frontiers in immunology · 2026
CCL22-producing macrophages are associated with Th1-related sweat duct inflammation in acquired idiopathic generalized anhidrosis.
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Takei Shingo, Hayashi Ryota, Okamura Manon, Katsumi Tatsuya, Kawai Toru, Abe Riichiro
Аннотация
BACKGROUND: Acquired idiopathic generalized anhidrosis (AIGA) is a rare disorder characterized by generalized loss of sweating without identifiable causes. Because few biomarkers reflect its underlying mechanisms, diagnosis at the initial visit is often difficult. Although steroid pulse therapy is widely used, approximately half of patients respond insufficiently. We therefore aimed to elucidate the immune mechanisms underlying AIGA and identify potential biomarkers for diagnosis and treatment response.
METHODS: Fourteen patients with AIGA affecting more than 25% of body surface area were enrolled after exclusion of secondary causes of anhidrosis. Serum levels of 40 cytokines and chemokines were quantified using a multiplex assay and correlated with clinical parameters. Skin biopsy specimens were analyzed by histology and immunohistochemistry to characterize inflammatory cell infiltration and identify cellular sources of selected mediators.
RESULTS: Inflammatory cell infiltration was consistently observed around sweat ducts, predominantly composed of CD4+ T cells. Serum profiling revealed significant elevations of CCL22 and IFN-γ in AIGA compared with healthy controls, with a strong positive correlation between them. Consistently, the downstream chemokine CXCL10 was also increased. Double immunostaining identified CD68+ macrophages as the main source of CCL22 in periductal regions. Serum Macrophage migration inhibitory factor (MIF) levels were significantly higher in steroid-resistant cases, whereas MIF expression within sweat ducts was markedly reduced, suggesting disruption of local immune privilege.
CONCLUSIONS: These findings suggest that AIGA involves a macrophage-CCL22-Th1-IFN-γ inflammatory axis associated with collapse of sweat duct immune privilege. Serum MIF may serve as a potential biomarker for predicting steroid responsiveness.
METHODS: Fourteen patients with AIGA affecting more than 25% of body surface area were enrolled after exclusion of secondary causes of anhidrosis. Serum levels of 40 cytokines and chemokines were quantified using a multiplex assay and correlated with clinical parameters. Skin biopsy specimens were analyzed by histology and immunohistochemistry to characterize inflammatory cell infiltration and identify cellular sources of selected mediators.
RESULTS: Inflammatory cell infiltration was consistently observed around sweat ducts, predominantly composed of CD4+ T cells. Serum profiling revealed significant elevations of CCL22 and IFN-γ in AIGA compared with healthy controls, with a strong positive correlation between them. Consistently, the downstream chemokine CXCL10 was also increased. Double immunostaining identified CD68+ macrophages as the main source of CCL22 in periductal regions. Serum Macrophage migration inhibitory factor (MIF) levels were significantly higher in steroid-resistant cases, whereas MIF expression within sweat ducts was markedly reduced, suggesting disruption of local immune privilege.
CONCLUSIONS: These findings suggest that AIGA involves a macrophage-CCL22-Th1-IFN-γ inflammatory axis associated with collapse of sweat duct immune privilege. Serum MIF may serve as a potential biomarker for predicting steroid responsiveness.