Cells & development · 2026
Samd7 is dispensable for spermatogenesis and male fertility in mice.
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Zhao Dan, Ma Xuan, Liu Yueqin, Wang Jinglin, Li Fan, Hua Rong, Zhang Chuanying, Wang Chang
Аннотация
BACKGROUND: Although many genes have been implicated in spermatogenesis, their precise relationships to male fertility remain unclear. Here, we investigated the in vivo importance of the highly conserved, testis-enriched gene Samd7 as a potential regulator of murine spermatogenesis and fertility.
METHODS: Samd7-/- mice were generated using CRISPR/Cas9. qPCR was used to assess Samd7 mRNA expression across mouse tissues. SAMD7 protein levels in testis were examined by Western blotting. Hematoxylin and eosin (H&E) staining of testis and epididymis was performed to evaluate spermatogenesis and sperm maturation. Immunofluorescence was used to characterize developmental stages of germ cells in the testis. Computer-assisted sperm analysis (CASA) was employed to measure sperm motility and related parameters.
RESULTS: Samd7 expression was enriched in testis beginning at postnatal week 3. Compared with wild-type (Samd7+/+) littermates, Samd7-/- males showed no abnormalities in sperm content, testicular morphology, or fertility. Histology confirmed intact seminiferous tubules and normal testicular architecture in both Samd7+/+ and Samd7-/- mice. No differences were detected in sperm morphology or motility, including swimming velocity, in Samd7-/- mice. Testes and epididymides from Samd7-/- animals contained the expected spectrum of germ cells-from spermatogonia to mature spermatozoa-consistent with unimpaired spermatogenesis.
CONCLUSIONS: These findings indicate that Samd7 is dispensable for murine spermatogenesis and male fertility under standard laboratory conditions.
METHODS: Samd7-/- mice were generated using CRISPR/Cas9. qPCR was used to assess Samd7 mRNA expression across mouse tissues. SAMD7 protein levels in testis were examined by Western blotting. Hematoxylin and eosin (H&E) staining of testis and epididymis was performed to evaluate spermatogenesis and sperm maturation. Immunofluorescence was used to characterize developmental stages of germ cells in the testis. Computer-assisted sperm analysis (CASA) was employed to measure sperm motility and related parameters.
RESULTS: Samd7 expression was enriched in testis beginning at postnatal week 3. Compared with wild-type (Samd7+/+) littermates, Samd7-/- males showed no abnormalities in sperm content, testicular morphology, or fertility. Histology confirmed intact seminiferous tubules and normal testicular architecture in both Samd7+/+ and Samd7-/- mice. No differences were detected in sperm morphology or motility, including swimming velocity, in Samd7-/- mice. Testes and epididymides from Samd7-/- animals contained the expected spectrum of germ cells-from spermatogonia to mature spermatozoa-consistent with unimpaired spermatogenesis.
CONCLUSIONS: These findings indicate that Samd7 is dispensable for murine spermatogenesis and male fertility under standard laboratory conditions.