Nan fang yi ke da xue xue bao = Journal of Southern Medical University · 2026
[Q-switched Nd:YAG 1064 nm laser combined with topical metformin lotion for treatment of chloasma in mice].
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Jiang Lu, Zhao Min, Liu Xing, Yang Hao, Mou Yunzhu
Аннотация
OBJECTIVES: To evaluate the efficacy and safety of Q-switched Nd:YAG 1064 nm laser and topical 30% metformin lotion for treatment of chloasma in mice and explore the possible therapeutic mechanism of topical metformin for chloasma.
METHODS: Forty female healthy Kunming mice were randomly divided into normal group (n=16) and chloasma model group (n=24), and the latter group was further divided into control, laser treatment, metformin lotion treatment, and laser with metformin (Laser+Met) treatment groups (n=6). Chloasma was induced in the mouse models by medium-wave ultraviolet irradiation and intramuscular injection of progesterone. After 4 weeks of treatment, melanocyte count, melanin and tyrosinase activities of the mice were detected using immunohistochemistry and Western blotting, and the mRNA expressions of CRE/MITF signaling pathway-related proteins were detected with quantitative real-time PCR.
RESULTS: The mouse models of chloasma receiving Laser+Met treatment had the lowest recurrence rate among the groups. After 4 weeks of treatment, melanocyte count and melanin and tyrosinase expressions, protein expressions of melanin and tyrosinase, and mRNA expressions of CREB1 and MITF in laser, metformin lotion, and Laser+Met groups were significantly reduced compared with those in the control group. Laser treatment and metformin lotion alone produced similar therapeutic effects in the mouse models, and the combined treatment achieved significantly better effects than either of them.
CONCLUSIONS: Q-switched Nd:YAG 1064 nm laser combined 30% metformin lotion effectively shortens the treatment course and reduces recurrence rate of chloasma in mice with a good safety profile. The therapeutic effect of metformin lotion is mediated possibly by regulation of the cAMP/MITF signaling pathway and reduction of melanin and tyrosinase synthesis.
METHODS: Forty female healthy Kunming mice were randomly divided into normal group (n=16) and chloasma model group (n=24), and the latter group was further divided into control, laser treatment, metformin lotion treatment, and laser with metformin (Laser+Met) treatment groups (n=6). Chloasma was induced in the mouse models by medium-wave ultraviolet irradiation and intramuscular injection of progesterone. After 4 weeks of treatment, melanocyte count, melanin and tyrosinase activities of the mice were detected using immunohistochemistry and Western blotting, and the mRNA expressions of CRE/MITF signaling pathway-related proteins were detected with quantitative real-time PCR.
RESULTS: The mouse models of chloasma receiving Laser+Met treatment had the lowest recurrence rate among the groups. After 4 weeks of treatment, melanocyte count and melanin and tyrosinase expressions, protein expressions of melanin and tyrosinase, and mRNA expressions of CREB1 and MITF in laser, metformin lotion, and Laser+Met groups were significantly reduced compared with those in the control group. Laser treatment and metformin lotion alone produced similar therapeutic effects in the mouse models, and the combined treatment achieved significantly better effects than either of them.
CONCLUSIONS: Q-switched Nd:YAG 1064 nm laser combined 30% metformin lotion effectively shortens the treatment course and reduces recurrence rate of chloasma in mice with a good safety profile. The therapeutic effect of metformin lotion is mediated possibly by regulation of the cAMP/MITF signaling pathway and reduction of melanin and tyrosinase synthesis.